Caspase 9 anticorps

N° du produit ABIN306797

L’anticorps anti-Caspase 9 Monoclonal Souris est utilisé pour la détection de Caspase 9 dans des échantillons de Humain. Il a été validé pour WB.

Aperçu rapide pour Caspase 9 anticorps (ABIN306797)

Antigène: Caspase 9 (CASP9) (Caspase 9, Apoptosis-Related Cysteine Peptidase (CASP9))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Caspase 9 est non-conjugé

Application: Western Blotting (WB)

Clone: LAAP6 96 2-22

Détail du produit anti-Caspase 9 anticorps

Fonction: Mouse anti Human Caspase-9

Réactivité croisée: Humain

Attributs du produit: Caspases are key effectors of programmed cell death. They are synthesized as inactive proenzymes which are activated by cleavage at a specific aspartate residue to form two subunits. These subunits are normally linked together by a linker which may be involved in the regulation of the different caspases. Caspase-9 is a member of the CED-3 family and bear high similarity to caspase-3. Procaspase-9 can be activated by either caspase-3 or granzyme B, although they cleave the proenzyme to different size subunits. Cleavage by granzyme B produces an active enzyme which is capable of cleaving PARP. Also, the ability of caspase-3 to activate caspase-9 seems to suggest that caspase-9 is further downstream of caspase-3 and may be involved in later changes in cells observed undergoing apoptosis.

Purification: Protein A/G Chromatography

Immunogène: Hybridoma produced by the fusion of splenocytes from mice immunized with recombinant human capase-9 protein and mouse myeloma cells.

Isotype: IgG

Information d'application

Indications d'application: Detects proenzyme form of human Caspase-9 by Western blot analysis as well as one of the cleavage products after treatment with thapsigargin. Optimal concentration should be evaluated by serial dilutions.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: phosphate buffered saline with 0.08 % sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles

Détails sur Caspase 9

Antigène: Caspase 9 (CASP9) (Caspase 9, Apoptosis-Related Cysteine Peptidase (CASP9))

Autre désignation: Caspase-9

Sujet: Cysteine-requiring Aspartate Protease-9, iCE-like apoptotic protease 6,Apoptotic protease Mch-6, Apoptotic protease-activating factor 3

UniProt: P55211

Pathways: Signalisation MAPK, Signalisation RTK, Apoptose, Caspase Cascade in Apoptosis, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Positive Regulation of Endopeptidase Activity