ERN1 anticorps (Internal Region)

N° du produit ABIN308413

Cet anticorps Chèvre Polyclonal détecte spécifiquement ERN1 dans WB, ELISA et FACS. Il présente une réactivité envers Humain.

Aperçu rapide pour ERN1 anticorps (Internal Region) (ABIN308413)

Antigène: ERN1 (Endoplasmic Reticulum To Nucleus Signaling 1 (ERN1))

Reactivité: Humain

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp ERN1 est non-conjugé

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS)

Classe de qualité: Verified

Détail du produit anti-ERN1 anticorps

Épitope: Internal Region

Fonction: ERN1 / IRE1a

Séquence: PNAHGKIKAM ISD

Réactivité croisée: Boeuf (Vache), Chien, Humain, Souris, Porc, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogène: Peptide with sequence C-PNAHGKIKAMISD, from the internal region of the protein sequence according to NP_001424.3.

Isotype: IgG

Information d'application

Indications d'application:

Western Blot: Approx 110 kDa band observed in Human Pancreas and Duodenum lysates (calculated MW of 110 kDa according to NP_001424.3). Recommended concentration: 0.1-0.3 μg/mL. Primary incubation 1 hour at room temperature.

Peptide ELISA: antibody detection limit dilution 1:16000.

Commentaires:

Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration: 10ug/ml.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Minimize freezing and thawing.

Stock: -20 °C

Stockage commentaire: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Détails sur ERN1

Antigène: ERN1 (Endoplasmic Reticulum To Nucleus Signaling 1 (ERN1))

Autre désignation: ERN1

Sujet: ERN1, IRE1, endoplasmic reticulum to nucleus signaling 1, FLJ30999, IRE1P, MGC163277, MGC163279, ER to nucleus signalling 1, endoplasmic reticulum to nucleus signalling 1, inositol-requiring 1, protein kinase/endoribonuclease, IRE1a, IRE1-alpha

ID gène: 2081, 78943, 498013

NCBI Accession: NP_001424

Pathways: ER-Nucleus Signaling, Unfolded Protein Response