ARFIP1 anticorps

N° du produit ABIN317818

Détail du produit anti-ARFIP1 anticorps

Antigène: ARFIP1 (ADP-Ribosylation Factor Interacting Protein 1 (ARFIP1))

Reactivité: Humain, Rat, Souris

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp ARFIP1 est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Specificité: This antibody detects endogenous levels of Arfaptin-1 protein.(region surrounding Lys314)

Purification: Affinity Chromatography using epitope-specific immunogen

Information d'application

Indications d'application: ELISA: 1: 40000approx. 1: 60000. WB: 1: 500approx. 1: 1000. IHC: 1: 50approx. 1: 200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH ~7.2, 0.05 % Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Détails sur ARFIP1

Antigène: ARFIP1 (ADP-Ribosylation Factor Interacting Protein 1 (ARFIP1))

Autre désignation: Arfaptin-1 (ARFIP1 Produits)

Synonymes: anticorps arfip1, anticorps MGC53116, anticorps DKFZp469C2322, anticorps HSU52521, anticorps 2310030I10Rik, anticorps AW123087, anticorps Arfaptin, anticorps D3Ertd598e, anticorps ADP ribosylation factor interacting protein 1, anticorps ADP ribosylation factor interacting protein 1 L homeolog, anticorps Arfaptin-1, anticorps ADP-ribosylation factor interacting protein 1, anticorps ARFIP1, anticorps arfip1.L, anticorps arfp1, anticorps Arfip1

Sujet: Arfaptin 1 is an ARF binding protein that inhibits PLD activation, vesicular trafficking and secretion. In the present report, we show that arfaptin 1 interacts with 'high speed' membranes independently of ARF. However, addition of myristoylated ARF3 (myrARF3) increases the association of arfaptin 1 with the membranes, suggesting that arfaptin 1 and ARF form a complex on the Golgi. Utilizing several deletion mutants of arfaptin 1 it is shown that the association of arfaptin 1 with myrARF3 is mediated via two binding sites on arfaptin 1. These two domains are needed for arfaptin 1 inhibition of PLD activation by myrARF3 in vitro.Synonyms: ADP-ribosylation factor-interacting protein 1, ARFIP1

Poids moléculaire: approx. 40 kDa

ID gène: 27236

NCBI Accession: NP_001020764

UniProt: P53367