HSP27 anticorps (pSer15)

N° du produit ABIN318042

Cet anticorps anti-HSP27 Polyclonal Lapin (ABIN318042) détecte spécifiquement HSP27 dans WB et IHC (p). L’anticorps est réactif avec des échantillons de Humain.

Aperçu rapide pour HSP27 anticorps (pSer15) (ABIN318042)

Antigène: HSP27 (HSPB1) (Heat Shock 27kDa Protein 1 (HSPB1))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp HSP27 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Détail du produit anti-HSP27 anticorps

Épitope: pSer15

Specificité: This antibody detects endogenous levels of HSP27 pSer15 protein.

Purification: Affinity Chromatography using epitope-specific immunogen.

Information d'application

Indications d'application: ELISA: 1/20000-1/40000. Western Blot: 1/500-1/1000. Immunohistochemistry: 1/50-1/200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH ~7.2 containing 0.05 % Sodium Azide as preservative.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Détails sur HSP27

Antigène: HSP27 (HSPB1) (Heat Shock 27kDa Protein 1 (HSPB1))

Autre désignation: HSPB1 / HSP27

Sujet: The heat shock proteins (HSPs) comprise a group of highly conserved, abundantly expressed proteins with diverse functions, including the assembly and sequestering of multiprotein complexes, transportation of nascent polypeptide chains across cellular membranes and regulation of protein folding. Heat shock proteins (also known as molecular chaperones) fall into six general families: HSP 90, HSP 70, HSP 60, the low molecular weight HSPs, the immunophilins and the HSP 110 family. The low molecular weight family includes HSP 10, HSP 20, HSP 27, HSP 32 and HSP 40. HSP 27 is a constitutively expressed cytoplasmic protein that co-localizes to the nucleus upon stress induced by insult. Heat, cytokines and hormones are among the factors that stimulate the synthesis of HSP 27. In vitro, HSP 27 becomes highly phosphorylated following exposure to stress. The discovery that HSP 27 is regulated by hormones such as estrogen has led to studies establishing a relationship between HSP 27 and breast cancer.Synonyms: 28 kDa heat shock protein, Estrogen-regulated 24 kDa protein, HSP25, HSP28, Heat shock 27 kDa protein, Heat shock protein beta-1, SRP27, Stress-responsive protein 27

Poids moléculaire: approx. 27 kDa

ID gène: 3315

NCBI Accession: NP_001531

UniProt: P04792

Pathways: Signalisation MAPK, Regulation of Actin Filament Polymerization, Signaling Events mediated by VEGFR1 and VEGFR2, Negative Regulation of intrinsic apoptotic Signaling, VEGF Signaling