c-MYC anticorps (C-Term) (Alkaline Phosphatase (AP))

N° du produit ABIN320077

Cet anticorps Souris Monoclonal détecte spécifiquement c-MYC dans WB, IHC (p), IHC (fro) et EIA. Il présente une réactivité avec des échantillons de Humain.

Aperçu rapide pour c-MYC anticorps (C-Term) (Alkaline Phosphatase (AP)) (ABIN320077)

Antigène: c-MYC (MYC) (Myc Proto-Oncogene protein (MYC))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp c-MYC est conjugé à/à la Alkaline Phosphatase (AP)

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA)

Clone: 9-00E-010

Détail du produit anti-c-MYC anticorps (Alkaline Phosphatase (AP))

Épitope: C-Term

Séquence: EQKLISEEDL

Specificité: This antibody detects the 62 kDa c-myc gene product, which is involved in the regulation of the cell cycle and cell growth. This antibody may also be used to detect the commonly used c-Myc 'Tag'.

Réactivité croisée (Details): Species reactivity (tested):Human.

Purification: Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant

Immunogène: Synthetic peptide sequence (AEEQKLISEEDLL) corresponding to the C-terminal region of Human c-myc. Spleen cells from immunised Balb/c mice were fused with cells of the myeloma cell line.

Isotype: IgG1

Information d'application

Indications d'application: ELISA: 1/500-1/5000. Western Blot: 1/100-1/500 (Non-Reducing Conditions). Detects a band of approximately 62 kDa in Colo 320HSR cell lysates. Immunohistochemistry on Frozen and Paraffin Sections: This product does not requireprotein digestion pre-treatment of paraffin sections prior to staining. It does not requireantigen retrieval using heat treatment prior to staining of paraffin sections.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL

Buffer: PBS, 0.09 % Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire: Store the antibody undiluted at 2-8 °C. DO NOT FREEZE!

Détails sur c-MYC

Antigène: c-MYC (MYC) (Myc Proto-Oncogene protein (MYC))

Autre désignation: C-Myc

Sujet: P62c-myc is primarily located to the cell nucleus, but has also been shown to localised to the cytoplasm in several cell lines. Overexpression of c-myc has been reported in a wide variety of human cancers.Synonyms: c-myc tag, myc tag, myc-tag

ID gène: 4609

NCBI Accession: NP_002458

UniProt: P01106

Pathways: Signalisation p53, Cycle Cellulaire, Sensory Perception of Sound, Transition Metal Ion Homeostasis, Mitotic G1-G1/S Phases, Positive Regulation of Endopeptidase Activity, Regulation of Carbohydrate Metabolic Process, Positive Regulation of Response to DNA Damage Stimulus, L'effet Warburg