TNF alpha anticorps (AA 77-197)

N° du produit ABIN3201324

L’anticorps anti-TNF alpha Monoclonal Souris est utilisé pour la détection de TNF alpha dans des échantillons de Macaque rhésus. Il a été validé pour WB, IHC, IP et ICC.

Aperçu rapide pour TNF alpha anticorps (AA 77-197) (ABIN3201324)

Antigène: TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))

Reactivité: Macaque rhésus

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp TNF alpha est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Clone: 1-1#

Détail du produit anti-TNF alpha anticorps

Épitope: AA 77-197

Fonction: Monoclonal Antibody to Tumor Necrosis Factor Alpha (TNFa)

Séquence: MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- VRSS SRTPSDKPVA HVVANPQAEG QLQWLNRRAN ALLANGVELT DNQLVVPSEG LYLIYSQVLF KGQGCPSNHV LLTHTISRIA VSYQTKVNLL SAIKSPCQRE TPEGAEAKPW YEPIYLGGVF QLEKGDRLSA EINLPDYLDF AESGQVYFGI IAL

Specificité: The antibody is a mouse monoclonal antibody raised against TNFa. It has been selected for its ability to recognize TNFa in immunohistochemical staining and western blotting.

Réactivité croisée: Humain

Purification: Protein A + Protein G affinity chromatography

Immunogène: Recombinant Tumor Necrosis Factor Alpha (TNFa) corresdonding to Val77~Gly197 Plus RGLSAGERVIDSALRSICPTISTLPSSGQV with N-terminal His Tag

Isotype: IgG2a kappa

Information d'application

Indications d'application: Western blotting: 0.5-2 μg/mL,Immunohistochemistry: 5-20 μg/mL,Immunocytochemistry: 5-20 μg/mL,Optimal working dilutions must be determined by end user.

Commentaires:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/mL

Buffer: 0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.

Agent conservateur: ProClin

Précaution d'utilisation: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Conseil sur la manipulation: Avoid repeated freeze/thaw cycles

Stock: 4 °C,-20 °C

Stockage commentaire: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Date de péremption: 24 months

Détails sur TNF alpha

Antigène: TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))

Autre désignation: TNFa

Sujet: DIF, TNF-A, TNFSF2, Cachectin, Tumor Necrosis Factor Ligand Superfamily Member 2

Pathways: Signalisation NF-kappaB, Apoptose, Caspase Cascade in Apoptosis, Signalisation TLR, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Inflammasome