TLR4 anticorps (AA 238-609)

N° du produit ABIN334262

L’anticorps Souris Monoclonal anti-TLR4 a été validé pour FACS, IHC, ELISA et IHC (p). Il convient pour détecter TLR4 dans des échantillons de Humain.

Aperçu rapide pour TLR4 anticorps (AA 238-609) (ABIN334262)

Antigène: TLR4 (Toll-Like Receptor 4 (TLR4))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp TLR4 est non-conjugé

Application: Flow Cytometry (FACS), Immunohistochemistry (IHC), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: 1E11

Détail du produit anti-TLR4 anticorps

Épitope: AA 238-609

Specificité: Recognizes human Toll-like Receptor 4 (TLR4).

Purification: Purified

Immunogène: Recombinant protein corresponding to amino acids 238-609 of human Toll-like Receptor 4 expressed in E. coli.

Type of Immunogen: Recombinant protein

Isotype: IgG2a

Information d'application

Indications d'application: Approved: ELISA (1:2000 - 1:4000), Flo, IHC, IHC-P (5 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.Flow Cytometry: 3 μg/10e6 cellsOptimal dilutions to be determined by researcher.

Commentaires:

Target Species of Antibody: Human

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: Lot specific

Buffer: Borate buffered saline,  pH 8.2. No preservatives or amine-containing buffer salts added.

Agent conservateur: Without preservative

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C,-20 °C

Stockage commentaire: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Détails sur TLR4

Antigène: TLR4 (Toll-Like Receptor 4 (TLR4))

Autre désignation: TLR4

Sujet: Name/Gene ID: TLR4
Family: Toll-like Receptor

Synonyms: TLR4, CD284, CD284 antigen, Homolog of Drosophila toll, TLR-4, TOLL, ARMD10, Toll-like receptor 4, HToll

ID gène: 7099

UniProt: O00206

Pathways: Signalisation TLR, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Toll-Like Receptors Cascades, Inflammasome, S100 Proteins