s100a4 anticorps (AA 86-98)

N° du produit ABIN337109

L’anticorps anti-s100a4 Polyclonal Chèvre est utilisé pour la détection de s100a4 dans des échantillons de Humain, Boeuf (Vache), Lapin, Chien, Cheval et Porc. Il a été validé pour WB, IHC, ELISA et IHC (p).

Aperçu rapide pour s100a4 anticorps (AA 86-98) (ABIN337109)

Antigène: s100a4 (S100A4) (S100 Calcium Binding Protein A4 (S100A4))

Reactivité: Humain, Boeuf (Vache), Lapin, Chien, Cheval, Porc

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp s100a4 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Détail du produit anti-s100a4 anticorps

Épitope: AA 86-98

Specificité: Human S100A4 / FSP1. Both variants (NP_002952.1, NP_062427.1) represent identical protein.

Homologie: Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Panda, Bovine, Dog, Horse, Rabbit, Pig (100%) Marmoset, Hamster, Elephant, Bat (92%) Mouse, Rat (85%).

Purification: Immunoaffinity purified

Immunogène: Synthetic peptide CNEFFEGFPDKQP from the C-terminus of human S100A4 / FSP1 (NP_002952.1, NP_062427.1). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Panda, Bovine, Dog, Horse, Rabbit, Pig (100%), Marmoset, Hamster, Elephant, Bat (92%), Mouse, Rat (85%).

Type of Immunogen: Synthetic peptide

Information d'application

Indications d'application: Approved: IHC, IHC-P (2.5 - 5 μg/mL), Peptide-ELISA (1:128000), WB (0.03 - 0.1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 2.5-5 μg/mL.

Commentaires:

Target Species of Antibody: Human

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Minimize freezing and thawing.

Stock: -20 °C

Stockage commentaire: Store at -20°C. Avoid freeze-thaw cycles.

Détails sur s100a4

Antigène: s100a4 (S100A4) (S100 Calcium Binding Protein A4 (S100A4))

Autre désignation: S100A4 / FSP1

Sujet: Name/Gene ID: S100A4

Synonyms: S100A4, 42A, 18A2, Fibroblast-specific protein-1, FSP1, MTS1, PEL98, Protein Mts1, Calvasculin, CAPL, Metastasin, p9KA, Protein S100-A4

ID gène: 6275

NCBI Accession: NP_002952, NP_062427

UniProt: P26447

Pathways: S100 Proteins