OGT anticorps (AA 543-555)

N° du produit ABIN337257

Cet anticorps anti-OGT Polyclonal Chèvre (ABIN337257) détecte spécifiquement OGT dans WB, ELISA, IHC et IHC (p). L’anticorps est réactif avec des échantillons de Humain, Souris, Rat, Chien, Boeuf (Vache), Cheval, Lapin, Roussette (Chauve-souris), Singe, Porc et Hamster.

Aperçu rapide pour OGT anticorps (AA 543-555) (ABIN337257)

Antigène: OGT (O-Linked N-Acetylglucosamine (GlcNAc) Transferase (UDP-N-Acetylglucosamine:polypeptide-N-Acetylglucosaminyl Transferase) (OGT))

Reactivité: Humain, Souris, Rat, Chien, Boeuf (Vache), Cheval, Lapin, Roussette (Chauve-souris), Singe, Porc, Hamster

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp OGT est non-conjugé

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Détail du produit anti-OGT anticorps

Épitope: AA 543-555

Specificité: Human OGT. This antibody is expected to recognise both reported isoforms (NP_858058.1 and NP_858059.1

Homologie: Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Dog, Bovine, Bat, Horse, Rabbit, Pig (100%) Xenopus (92%) Turkey, Chicken, Pufferfish, Zebrafish (85%).

Purification: Immunoaffinity purified

Immunogène: Synthetic peptide C-YEHPKDLKLSDGR from an internal region of human OGT (NP_858058.1, NP_858059.1). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Dog, Bovine, Bat, Horse, Rabbit, Pig (100%), Xenopus (92%), Turkey, Chicken, Pufferfish, Zebrafish (85%).

Type of Immunogen: Synthetic peptide

Information d'application

Indications d'application: Approved: IHC, IHC-P (5 μg/mL), Peptide-ELISA (1:64000), WB (1:64000, 0.05 - 0.2 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Commentaires:

Target Species of Antibody: Human

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Minimize freezing and thawing.

Stock: -20 °C

Stockage commentaire: Store at -20°C. Avoid freeze-thaw cycles.

Détails sur OGT

Antigène: OGT (O-Linked N-Acetylglucosamine (GlcNAc) Transferase (UDP-N-Acetylglucosamine:polypeptide-N-Acetylglucosaminyl Transferase) (OGT))

Autre désignation: OGT / O-GLCNAC

Sujet: Name/Gene ID: OGT

Synonyms: OGT, HRNT1, O-GLCNAC

ID gène: 8473

NCBI Accession: NP_858058, NP_858059

UniProt: O15294

Pathways: Regulation of Carbohydrate Metabolic Process