PGBD1 anticorps (AA 230-241)

N° du produit ABIN342993

Détail du produit anti-PGBD1 anticorps

Antigène: PGBD1 (PiggyBac Transposable Element Derived 1 (PGBD1))

Épitope: AA 230-241

Reactivité: Humain

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp PGBD1 est non-conjugé

Application: ELISA

Specificité: Human PGBD1.

Homologie: Percent identity with other species by BLAST analysis: Human (100%), Gorilla (92%), Gibbon (92%), Orangutan (83%), Monkey (83%).

Purification: Immunoaffinity purified

Immunogène: Synthetic peptide C-EKWSHLSLTRRN from an internal region of human PGBD1 (NP_115896.1). Percent identity with other species by BLAST analysis: Human (100%), Gorilla (92%), Gibbon (92%), Orangutan (83%), Monkey (83%).

Type of Immunogen: Synthetic peptide

Information d'application

Indications d'application: Approved: Peptide-ELISA (1:4000)

Usage: Peptide ELISA: antibody detection limit dilution 1:4000. Western Blot: Preliminary experiments gave bands at approx 150kD and 17kD in lysates of cell lines HeLa, HepG2 and K562 after 0.5 μg/mL antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 92.5kD according to NP_115896.1. Both detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands). We would appreciate any feedback from people in the field - have any results been reported with other antibodies / lysates? Have any further splice variants / modified forms been reported?

Not recommended for: IHC-P

Commentaires:

Target Species of Antibody: Human

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Minimize freezing and thawing.

Stock: -20 °C

Stockage commentaire: Store at -20°C. Avoid freeze-thaw cycles.

Détails sur PGBD1

Antigène: PGBD1 (PiggyBac Transposable Element Derived 1 (PGBD1))

Autre désignation: PGBD1 (PGBD1 Produits)

Synonymes: anticorps 4921509E05Rik, anticorps PGBD1, anticorps DKFZp469J1439, anticorps HUCEP-4, anticorps SCAND4, anticorps dJ874C20.4, anticorps piggyBac transposable element derived 1, anticorps PGBD1, anticorps Pgbd1

Sujet: Name/Gene ID: PGBD1

Synonyms: PGBD1, Cerebral protein 4, Cerebral protein-4, DJ874C20.4, HUCEP-4, SCAND4

ID gène: 84547

NCBI Accession: NP_115896

UniProt: Q96JS3