CD14 anticorps

N° du produit ABIN343172

Il existe 4+ publications pour ce produit. L’anticorps anti-CD14 Monoclonal Souris est utilisé pour la détection de CD14 dans des échantillons de Humain. Il a été validé pour FACS, WB, ELISA et Func.

Aperçu rapide pour CD14 anticorps (ABIN343172)

Antigène: CD14

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp CD14 est non-conjugé

Application: Flow Cytometry (FACS), Western Blotting (WB), ELISA, Functional Studies (Func)

Clone: BiG 7

Détail du produit anti-CD14 anticorps

Purification: Protein G purified

Immunogène: Monocytes and immunoaffinity purified soluble CD14

Isotype: IgG1

Information d'application

Indications d'application: Suitable for immunostaining of CD14 positive cells, (Flow cytometry), ELISA, Suitable for Western blot (non reducing). Non inhibition of LPS binding to CD14, binding epitope: amino acid 9-13, 39-44, Binding titre: 1:10.000

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Concentration: 1 mg/ml

Buffer: 0.1 mg/100 ul PBS without NaN3

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Références pour anticorps anti-CD14 (ABIN343172)

Muansson, Kjaell, Pellett, Nagy, Welch, Baeckhed, Frisan, Richter-Dahlfors: "Role of the lipopolysaccharide-CD14 complex for the activity of hemolysin from uropathogenic Escherichia coli." dans: Infection and immunity, Vol. 75, Issue 2, pp. 997-1004, (2007) (PubMed).

Neilsen, Zimmerman, McIntyre: "Escherichia coli Braun lipoprotein induces a lipopolysaccharide-like endotoxic response from primary human endothelial cells." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 167, Issue 9, pp. 5231-9, (2001) (PubMed).

Fan, Stelter, Menzel, Jack, Spreitzer, Hartung, Schuett: "Structures in Bacillus subtilis are recognized by CD14 in a lipopolysaccharide binding protein-dependent reaction." dans: Infection and immunity, Vol. 67, Issue 6, pp. 2964-8, (1999) (PubMed).

Dziarski, Tapping, Tobias: "Binding of bacterial peptidoglycan to CD14." dans: The Journal of biological chemistry, Vol. 273, Issue 15, pp. 8680-90, (1998) (PubMed).

Détails sur CD14

Antigène: CD14

Autre désignation: CD14

Sujet: BiG 7 represents an excellent marker for CD14. The CD14 glycoprotein, gp 55, is present on most monocytic and macrophages like cell types: monocytes, macrophages, Kupffer cells, pleural phagocytic cells and dendritic reticular cells. CD14 is also observed on granulocytes and activated or transformed B-cells. Furthermore CD14 is present in a soluble form in human serum, urine and other body fluids. The CD14 molecule has been reported to be a receptor for endotoxin.

Pathways: Signalisation TLR, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Toll-Like Receptors Cascades