EGFR anticorps (pTyr1173)

N° du produit ABIN343717

Cet anticorps Souris Monoclonal détecte spécifiquement EGFR dans WB, IP et ICFC. Il présente une réactivité envers Humain et a été mentionné dans 1 publication.

Aperçu rapide pour EGFR anticorps (pTyr1173) (ABIN343717)

Antigène: EGFR (Epidermal Growth Factor Receptor (EGFR))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp EGFR est non-conjugé

Application: Western Blotting (WB), Immunoprecipitation (IP), Intracellular Flow Cytometry (ICFC)

Clone: EM-13

Détail du produit anti-EGFR anticorps

Épitope: pTyr1173

Fonction: Anti-Hu EGFR (pY1173) Purified

Specificité: The mouse monoclonal antibody EM-13 reacts with human EGFR (ErbB1 / HER1) phosphorylated on intracellular tyrosine 1173.

Réactivité croisée (Details): Human

Purification: Purified by protein-A affinity chromatography.

Pureté: > 95 % (by SDS-PAGE)

Immunogène: Synthetic phospho-peptide covering sequence around tyrosine 1173 of human EGFR.

Isotype: IgG1

Information d'application

Indications d'application: Western blotting: Recommended dilution: 1 μg/mL, positive control: EGF-stimulated A431, negative control: non-stimulated A431.
Flow cytometry: Recommended dilution: 1-4 μg/mL. Intracellular staining.

Restrictions: For Research Use only

Stockage

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Do not freeze.

Stock: 4 °C

Stockage commentaire: Store at 2-8°C. Do not freeze.

Références pour anticorps anti-EGFR (ABIN343717)

Shlepova, Bychkov, Shipunova, Shramova, Shulepko, Gornostaeva, Kiseleva, Kukushkin, Kazakov, Tukhovskaya, Dyachenko, Murashev, Shenkarev, Deyev, Kirpichnikov, Lyukmanova: "Combination with a Low Dose of Doxorubicin Further Boosts the Antitumor Effect of SLURP-1 In Vivo and Associates with EGFR Down-Regulation." dans: Acta naturae, Vol. 17, Issue 1, pp. 87-96, (2025) (PubMed).

Détails sur EGFR

Antigène: EGFR (Epidermal Growth Factor Receptor (EGFR))

Autre désignation: EGFR

Sujet: Epidermal growth factor receptor,The oncoprotein EGFR (epidermal growth factor receptor), also known as HER1 / ErbB1, is a member of ErbB family of cell surface receptor tyrosine kinases. This 170 kDa transmembrane glycoprotein is often associated with cancerogenesis, although its activation state is controlled at various levels including trafficking and degradation steps. Binding of receptor-specific ligands to the EGFR ectodomain results in formation of homodimeric or heterodimeric kinase-active complexes into which HER2 / ErbB2 is recruited as a preferred partner. Subsequent signaling cascades such as RAS/MAPK and PI3K/AKT pathways lead to cell proliferation and survival responses.,EGFR, EGF-R, Her1, ERBB1, PIG61, NISBD2

ID gène: 1956

UniProt: P00533

Pathways: Signalisation NF-kappaB, Signalisation RTK, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Stem Cell Maintenance, Hepatitis C, Positive Regulation of Response to DNA Damage Stimulus, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, EGFR Downregulation, S100 Proteins