ERCC3 anticorps (AA 242-261)

N° du produit ABIN351537

Cet anticorps anti-ERCC3 est un anticorps Souris Monoclonal détectant ERCC3 dans WB, ELISA, IHC, IP et IHC (p). Adapté pour Humain.

Aperçu rapide pour ERCC3 anticorps (AA 242-261) (ABIN351537)

Antigène: ERCC3 (DNA Repair Protein Complementing XP-B Cells (ERCC3))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp ERCC3 est non-conjugé

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: 3G4

Détail du produit anti-ERCC3 anticorps

Épitope: AA 242-261

Marque: IHC-plus™

Specificité: Recognizes an epitope located between aa242-261 of ERCC-3 (largest subunit of TFIIH).

Purification: Protein G purified

Immunogène: Synthetic peptide corresponding to aa242-261 of human ERCC-3.

Type of Immunogen: Synthetic peptide

Isotype: IgG2b

Information d'application

Indications d'application: Approved: ELISA, IHC, IHC-P (5 - 10 μg/mL), IP, WB

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5-10 μg/mL. Immunoprecipitation: Immunoprecipitates transcriptionally active TFIIH in a reconstituted assay.

Commentaires:

Target Species of Antibody: Human

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: Lot specific

Buffer: 0.1 M Tris-HCl,  pH 7.5. Sourced from ascites.

Conseil sur la manipulation: Avoid repeat freeze-thaw cycles.

Stock: 4 °C,-20 °C

Stockage commentaire: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Détails sur ERCC3

Antigène: ERCC3 (DNA Repair Protein Complementing XP-B Cells (ERCC3))

Autre désignation: ERCC3 / XPB

Sujet: Name/Gene ID: ERCC3

Synonyms: ERCC3, BTF2, GTF2H, RAD25, TFIIH p89, TFIIH, XPB, BTF2 p89, XPBC, TFIIH 89 kDa subunit

ID gène: 2071

UniProt: P19447

Pathways: Réparation de l'ADN