RAF1 anticorps (pSer259)

N° du produit ABIN361883

Il existe 1 publication pour ce produit. L’anticorps anti-RAF1 Polyclonal Lapin est utilisé pour la détection de RAF1 dans des échantillons de Humain, Souris et Rat. Il a été validé pour WB et IHC.

Aperçu rapide pour RAF1 anticorps (pSer259) (ABIN361883)

Antigène: RAF1 (V-Raf-1 Murine Leukemia Viral Oncogene Homolog 1 (RAF1))

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp RAF1 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Détail du produit anti-RAF1 anticorps

Épitope: pSer259

Specificité: The antibody detects endogenous level of Raf-1 only when phosphorylated at serine 259.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.

Immunogène: Peptide sequence around phosphorylation site of pSer259 (S-T-S (p) -T-P) derived from Human Raf1. Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates.

Isotype: IgG

Information d'application

Indications d'application: Western blotting: 1:500-1:1000
Immunohistochemistry: 1:50-1:100

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C/-20 °C

Stockage commentaire: Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.

Références pour anticorps anti-RAF1 (ABIN361883)

Qiu, Wang, Han, Liu, Huang, Altaf, Qiu, Javed, Zheng, Chen, Li: "Prognostic impact of Raf-1 and p-Raf-1 expressions for poor survival rate in non-small cell lung cancer." dans: Cancer science, Vol. 103, Issue 10, pp. 1774-9, (2012) (PubMed).

Détails sur RAF1

Antigène: RAF1 (V-Raf-1 Murine Leukemia Viral Oncogene Homolog 1 (RAF1))

Autre désignation: Raf1

Sujet: Involved in the transduction of mitogenic signals from the cell membrane to the nucleus. Part of the Ras-dependent signaling pathway from receptors to the nucleus. Protects cells from apoptosis mediated by STK3.

Poids moléculaire: 73 kDa

UniProt: P04049

Pathways: Signalisation MAPK, Signalisation RTK, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, cAMP Metabolic Process, Stem Cell Maintenance, Hepatitis C, Autophagy, Signaling of Hepatocyte Growth Factor Receptor, VEGF Signaling, BCR Signaling