ATF2 anticorps (pThr55, pThr73)

N° du produit ABIN361936

Cet anticorps anti-ATF2 Polyclonal Lapin (ABIN361936) détecte spécifiquement ATF2 dans IHC et IF. L’anticorps est réactif avec des échantillons de Humain, Souris et Rat.

Aperçu rapide pour ATF2 anticorps (pThr55, pThr73) (ABIN361936)

Antigène: ATF2 (Activating Transcription Factor 2 (ATF2))

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp ATF2 est non-conjugé

Application: Immunohistochemistry (IHC), Immunofluorescence (IF)

Détail du produit anti-ATF2 anticorps

Épitope: pThr55, pThr73

Specificité: The antibody detects endogenous level of ATF-2 only whenphosphorylated at threonine 73 or 55.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific phosphopeptide. The antibody against non-phosphopeptide was removedby chromatography using non-phosphopeptide corresponding to the phosphorylation site.

Immunogène: Peptide sequence around phosphorylation site of pThr73 or 55 (T-P-T (p) -R-F) derived from Human ATF2. Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates.

Isotype: IgG

Information d'application

Indications d'application: Western blotting: 1:500-1:1000
Immunohistochemistry: 1:50-1:100
Immunofluorescence: 1:100-1:200

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C/-20 °C

Stockage commentaire: Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.

Détails sur ATF2

Antigène: ATF2 (Activating Transcription Factor 2 (ATF2))

Autre désignation: ATF2

Sujet: Transcriptional activator, probably constitutive, which binds to the cAMP-responsive element (CRE) (consensus: 5'-GTGACGT[AC][AG]-3'), a sequence present in many viral and cellular promoters. Interaction with JUN redirects JUN to bind to CRES preferentially over the 12-O-tetradecanoylphorbol-13-acetate response elements (TRES) as part of an ATF2-c-Jun complex.

Poids moléculaire: 65-75 kDa

NCBI Accession: NP_001871

UniProt: P15336

Pathways: Signalisation MAPK, Signalisation RTK, Thyroid Hormone Synthesis, Activation of Innate immune Response, Chromatin Binding, Myometrial Relaxation and Contraction, Synaptic Membrane, Tube Formation, Toll-Like Receptors Cascades