FOXO1 anticorps (pSer256)

N° du produit ABIN362087

Il existe 3+ publications pour ce produit. L’anticorps anti-FOXO1 Polyclonal Lapin est utilisé pour la détection de FOXO1 dans des échantillons de Humain, Souris et Rat. Il a été validé pour WB, IHC et IF.

Aperçu rapide pour FOXO1 anticorps (pSer256) (ABIN362087)

Antigène: FOXO1 (Forkhead Box O1 (FOXO1))

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp FOXO1 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Détail du produit anti-FOXO1 anticorps

Épitope: pSer256

Specificité: The antibody detects endogenous level of FKHR only when phosphorylated at serine 256.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific phosphopeptide. The antibody against non-phosphopeptide was removedby chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.

Immunogène: Peptide sequence around phosphorylation site of pSer256 (A-A-S (p) -M-D) derived from Human FKHR. Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates.

Isotype: IgG

Information d'application

Indications d'application: Western blotting: 1:500-1:1000
Immunohistochemistry: 1:50-1:100
Immunofluorescence: 1:100-1:200

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C/-20 °C

Stockage commentaire: Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.

Références pour anticorps anti-FOXO1 (ABIN362087)

Pierce, Podlutskaya, Halloran, Hussong, Lin, Burbank, Hart, Galvan: "Over-expression of heat shock factor 1 phenocopies the effect of chronic inhibition of TOR by rapamycin and is sufficient to ameliorate Alzheimer's-like deficits in mice modeling the disease." dans: Journal of neurochemistry, Vol. 124, Issue 6, pp. 880-93, (2013) (PubMed).

Ma, Li, Wang, Gong, Zhang, Li, Ji, Liu, Chen, Xiang: "Atrial natriuretic peptide suppresses Th17 development through regulation of cGMP-dependent protein kinase and PI3K-Akt signaling pathways." dans: Regulatory peptides, Vol. 181, pp. 9-16, (2013) (PubMed).

Zhan, Wang, Li, Xu, Sun, Xu: "Activation of Akt/FoxO signaling pathway contributes to induction of neuroprotection against transient global cerebral ischemia by hypoxic pre-conditioning in adult rats." dans: Journal of neurochemistry, Vol. 114, Issue 3, pp. 897-908, (2010) (PubMed).

Détails sur FOXO1

Antigène: FOXO1 (Forkhead Box O1 (FOXO1))

Autre désignation: FKHR

Sujet: Transcription factor.

Poids moléculaire: 78-82 kDa

NCBI Accession: NP_002006

UniProt: Q12778

Pathways: Signalisation PI3K-Akt, Cycle Cellulaire, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Carbohydrate Homeostasis, Chromatin Binding, Regulation of Carbohydrate Metabolic Process, CXCR4-mediated Signaling Events, BCR Signaling