CDC25C anticorps (pSer216)

N° du produit ABIN362094

L’anticorps anti-CDC25C Polyclonal Lapin est utilisé pour la détection de CDC25C dans des échantillons de Humain. Il a été validé pour IHC et IF.

Aperçu rapide pour CDC25C anticorps (pSer216) (ABIN362094)

Antigène: CDC25C (Cell Division Cycle 25 Homolog C (S. Pombe) (CDC25C))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp CDC25C est non-conjugé

Application: Immunohistochemistry (IHC), Immunofluorescence (IF)

Détail du produit anti-CDC25C anticorps

Épitope: pSer216

Specificité: The antibody detects endogenous level of cdc25C only when phosphorylated at serine 216.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific phosphopeptide. The antibody against non-phosphopeptide was removedby chromatography using non-phosphopeptide corresponding to the phosphorylation site.

Immunogène: Peptide sequence around phosphorylation site of pSer216 (S-P-S (p) -M-P) derived from Human cdc25C. Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates.

Isotype: IgG

Information d'application

Indications d'application: Western blotting: 1:500-1:1000
Immunohistochemistry: 1:50-1:100
Immunofluorescence: 1:100-1:200

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C/-20 °C

Stockage commentaire: Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.

Détails sur CDC25C

Antigène: CDC25C (Cell Division Cycle 25 Homolog C (S. Pombe) (CDC25C))

Autre désignation: cdc25C

Sujet: Cdc25C is highly conserved during evolution and it plays a key role in the regulation of cell division. The encoded protein is a tyrosine phosphatase and belongs to the Cdc25 phosphatase family. It directs dephosphorylation of cyclin B-bound CDC2 and triggers entry into mitosis. It is also thought to suppress p53-induced growth arrest. Multiple alternatively spliced transcript variants of this gene have been described, however, the full-length nature of many of them is not known.

Poids moléculaire: 80 kDa

NCBI Accession: NP_001781

UniProt: P30307

Pathways: Cycle Cellulaire, M Phase