PAK1 anticorps (pThr212)

N° du produit ABIN362156

Détail du produit anti-PAK1 anticorps

Antigène: PAK1 (P21-Activated Kinase 1 (PAK1))

Épitope: pThr212

Reactivité: Humain, Rat, Souris

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp PAK1 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Specificité: The antibody detects endogenous level of PAK1 only when phosphorylated at threonine 212.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific phosphopeptide. The antibody against non-phosphopeptide was removedby chromatography using non-phosphopeptide corresponding to the phosphorylation site.

Immunogène: Peptide sequence around phosphorylation site of pThr212 (P-V-T (p) -P-T) derived from Human PAK1. Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates.

Isotype: IgG

Information d'application

Indications d'application: Western blotting: 1:500-1:1000
Immunohistochemistry: 1:50-1:100
Immunofluorescence: 1:100-1:200

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C/-20 °C

Stockage commentaire: Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.

Détails sur PAK1

Antigène: PAK1 (P21-Activated Kinase 1 (PAK1))

Autre désignation: PAK1 (PAK1 Produits)

Synonymes: anticorps X-PAK1, anticorps XPak1, anticorps pak, anticorps pak-1, anticorps pakalpha, anticorps PAKalpha, anticorps AW045634, anticorps PAK-1, anticorps Paka, anticorps p21 protein (Cdc42/Rac)-activated kinase 1 S homeolog, anticorps p21 (RAC1) activated kinase 1, anticorps p21 protein (Cdc42/Rac)-activated kinase 1, anticorps Serine/threonine-protein kinase pak-1, anticorps pak1.S, anticorps PAK1, anticorps Pak1, anticorps pak-1

Sujet: The activated kinase acts on a variety of targets. Likely to be the GTPase effector that links the Rho-related GTPases to the JNK MAP kinase pathway. Activated by CDC42 and RAC1. Involved in dissolution of stress fibers and reorganization of focal complexes. Involved in regulation of microtubule biogenesis through phosphorylation of TBCB. Activity is inhibited in cells undergoing apoptosis, potentially due to binding of CDC2L1 and CDC2L2.

Poids moléculaire: 68 kDa

NCBI Accession: NP_001122

UniProt: Q13153

Pathways: Signalisation MAPK, Signalisation RTK, TCR Signaling, Fc-epsilon Receptor Signaling Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Skeletal Muscle Fiber Development, CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, Signaling of Hepatocyte Growth Factor Receptor, Embryonic Body Morphogenesis