MEK1 anticorps (pSer221)

N° du produit ABIN362167

Cet anticorps Lapin Polyclonal détecte spécifiquement MEK1 dans WB, IHC et IF. Il présente une réactivité avec des échantillons de Humain, Souris et Rat.Il a été cité dans 1 publication.

Aperçu rapide pour MEK1 anticorps (pSer221) (ABIN362167)

Antigène: MEK1 (MAP2K1) (Mitogen-Activated Protein Kinase Kinase 1 (MAP2K1))

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp MEK1 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Détail du produit anti-MEK1 anticorps

Épitope: pSer221

Specificité: The antibody detects endogenous level of MEK1 only when phosphorylated at serine 221.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific phosphopeptide. The antibody against non-phosphopeptide was removedby chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.

Immunogène: Peptide sequence around phosphorylation site of pSer221 (A-N-S (p) -F-V) derived from Human MEK1. Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates.

Isotype: IgG

Information d'application

Indications d'application: Western blotting: 1:500-1:1000
Immunohistochemistry: 1:50-1:100
Immunofluorescence: 1:100-1:200

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C/-20 °C

Stockage commentaire: Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.

Références pour anticorps anti-MEK1 (ABIN362167)

Ikari, Atomi, Kinjo, Sasaki, Sugatani: "Magnesium deprivation inhibits a MEK-ERK cascade and cell proliferation in renal epithelial Madin-Darby canine kidney cells." dans: Life sciences, Vol. 86, Issue 19-20, pp. 766-73, (2010) (PubMed).

Détails sur MEK1

Antigène: MEK1 (MAP2K1) (Mitogen-Activated Protein Kinase Kinase 1 (MAP2K1))

Autre désignation: MEK1

Sujet: Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in a Thr-Glu-Tyr sequence located in MAP kinases. Activates ERK1 and ERK2 MAP kinases.

Poids moléculaire: 45 kDa

NCBI Accession: NP_002746

UniProt: Q02750

Pathways: Signalisation MAPK, Signalisation RTK, Interferon-gamma Pathway, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Toll-Like Receptors Cascades, Autophagy, Signaling of Hepatocyte Growth Factor Receptor, BCR Signaling