HSP27 anticorps (pSer15)

N° du produit ABIN362172

L’anticorps Lapin Polyclonal anti-HSP27 a été validé pour WB, IHC et IF. Il convient pour détecter HSP27 dans des échantillons de Humain. Il y a 1 publication disponible.

Aperçu rapide pour HSP27 anticorps (pSer15) (ABIN362172)

Antigène: HSP27 (HSPB1) (Heat Shock 27kDa Protein 1 (HSPB1))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp HSP27 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Détail du produit anti-HSP27 anticorps

Épitope: pSer15

Specificité: The antibody detects endogenous level of HSP27only when phosphorylated at serine 15.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific phosphopeptide. The antibody against non-phosphopeptide was removedby chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.

Immunogène: Peptide sequence around phosphorylation site of pSer15 (G-P-S (p) -W-D) derived from Human HSP27. Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates.

Isotype: IgG

Information d'application

Indications d'application: Western blotting: 1:500-1:1000
Immunohistochemistry: 1:50-1:100
Immunofluorescence: 1:100-1:200

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C/-20 °C

Stockage commentaire: Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.

Références pour anticorps anti-HSP27 (ABIN362172)

Park, Kudo, Hagiwara, Tabuchi, Watanabe, Munakata, Sakurai: "p38MAPK suppresses chronic pancreatitis by regulating HSP27 and BAD expression." dans: Free radical biology & medicine, Vol. 52, Issue 11-12, pp. 2284-91, (2012) (PubMed).

Détails sur HSP27

Antigène: HSP27 (HSPB1) (Heat Shock 27kDa Protein 1 (HSPB1))

Autre désignation: HSP27

Sujet: Involved in stress resistance and actin organization.

Poids moléculaire: 27 kDa

NCBI Accession: NP_001531

UniProt: P04792

Pathways: Signalisation MAPK, Regulation of Actin Filament Polymerization, Signaling Events mediated by VEGFR1 and VEGFR2, Negative Regulation of intrinsic apoptotic Signaling, VEGF Signaling