HSP27 anticorps (pSer82)
Aperçu rapide pour HSP27 anticorps (pSer82) (ABIN362306)
Antigène
Voir toutes HSP27 (HSPB1) AnticorpsReactivité
Hôte
Clonalité
Conjugué
Application
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Épitope
- pSer82
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Specificité
- The antibody detects endogenous level of HSP27 only when phosphorylated at serine 82.
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Purification
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
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Immunogène
- Peptide sequence around phosphorylation site of pSer82 (Q-L-S (p) -S-G) derived from Human HSP27. Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates.
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Isotype
- IgG
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Indications d'application
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Western blotting: 1:500-1:1000
Immunohistochemistry: 1:50-1:100 -
Restrictions
- For Research Use only
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Format
- Liquid
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Concentration
- 1 mg/mL
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Buffer
- Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
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Agent conservateur
- Sodium azide
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Précaution d'utilisation
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Stock
- 4 °C/-20 °C
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Stockage commentaire
- Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.
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- HSP27 (HSPB1) (Heat Shock 27kDa Protein 1 (HSPB1))
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Autre désignation
- HSP27
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Sujet
- Involved in stress resistance and actin organization.
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Poids moléculaire
- 27 kDa
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NCBI Accession
- NP_001531
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UniProt
- P04792
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Pathways
- Signalisation MAPK, Regulation of Actin Filament Polymerization, Signaling Events mediated by VEGFR1 and VEGFR2, Negative Regulation of intrinsic apoptotic Signaling, VEGF Signaling
Antigène
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