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HSP27 anticorps (pSer82)

HSPB1 Reactivité: Humain WB, IHC Hôte: Lapin Polyclonal unconjugated

N° du produit ABIN362306

Fiche de données

Antigène Voir toutes HSP27 (HSPB1) Anticorps

HSP27 (HSPB1) (Heat Shock 27kDa Protein 1 (HSPB1))
Épitope
42

37

27

16

16

10

8

8

7

5

3

3

3

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
pSer82
Reactivité
299

104

90

24

14

14

13

12

11

6

6

4

3

2

2

2

1
Humain
Hôte
237

66
Lapin
Anticorps secondaires appropriés
Clonalité
206

96
Polyclonal
Conjugué
138

23

17

13

12

8

7

7

7

7

7

7

4

4

4

4

4

4

4

3

3

3

3

2

1

1

1

1

1

1

1

1
Cet anticorp HSP27 est non-conjugé
Application
256

103

103

95

69

49

49

46

41

40

16

12

10

7

7

2

2

2

2

2

2

1

1

1

1
Western Blotting (WB), Immunohistochemistry (IHC)

Specificité

The antibody detects endogenous level of HSP27 only when phosphorylated at serine 82.

Purification

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

Immunogène

Peptide sequence around phosphorylation site of pSer82 (Q-L-S (p) -S-G) derived from Human HSP27. Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates.

Isotype

IgG

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anti-Heat Shock 27kDa Protein 1 (HSPB1) antibody (HRP)
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Indications d'application

Western blotting: 1:500-1:1000
Immunohistochemistry: 1:50-1:100

Restrictions

For Research Use only
Format

Liquid

Concentration

1 mg/mL

Buffer

Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Agent conservateur

Sodium azide

Précaution d'utilisation

This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock

4 °C/-20 °C

Stockage commentaire

Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.
Antigène

HSP27 (HSPB1) (Heat Shock 27kDa Protein 1 (HSPB1))

Autre désignation

HSP27 (HSPB1 Produits)

Synonymes

anticorps CMT2F, anticorps HMN2B, anticorps HS.76067, anticorps HSP27, anticorps HSP28, anticorps Hsp25, anticorps SRP27, anticorps 27kDa, anticorps Hsp27, anticorps cb153, anticorps cb660, anticorps hsp1, anticorps hsp25, anticorps hsp27, anticorps id:ibd2821, anticorps sb:cb660, anticorps zgc:103437, anticorps HSPB1, anticorps cmt2f, anticorps hs.76067, anticorps hsp28, anticorps heat shock protein family B (small) member 1, anticorps heat shock protein 1, anticorps heat shock protein, alpha-crystallin-related, 1, anticorps heat shock protein family B (small) member 1 L homeolog, anticorps HSPB1, anticorps Hspb1, anticorps hspb1, anticorps hspb1.L

Sujet

Involved in stress resistance and actin organization.

Poids moléculaire

27 kDa

NCBI Accession

NP_001531

UniProt

P04792

Pathways

Signalisation MAPK, Regulation of Actin Filament Polymerization, Signaling Events mediated by VEGFR1 and VEGFR2, Negative Regulation of intrinsic apoptotic Signaling, VEGF Signaling