H2AFX anticorps (pSer139)

N° du produit ABIN362340

Il existe 1 publication pour ce produit. L’anticorps anti-H2AFX Polyclonal Lapin est utilisé pour la détection de H2AFX dans des échantillons de Humain. Il a été validé pour WB et IF.

Aperçu rapide pour H2AFX anticorps (pSer139) (ABIN362340)

Antigène: H2AFX (H2A Histone Family, Member X (H2AFX))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp H2AFX est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF)

Détail du produit anti-H2AFX anticorps

Épitope: pSer139

Specificité: The antibody detects endogenous level of Histone H2A.X only when phosphorylated at serine 139.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific phosphopeptide. The antibody against non-phosphopeptide was removedby chromatography using non-phosphopeptide corresponding to the phosphorylation site.

Immunogène: Peptide sequence around phosphorylation site of pSer139 (Q-A-S (p) -Q-E) derived from Human Histone H2A.X. Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates.

Isotype: IgG

Information d'application

Indications d'application: Western blotting: 1:500-1:1000
Immunofluorescence: 1:100-1:200

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C/-20 °C

Stockage commentaire: Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.

Références pour anticorps anti-H2AFX (ABIN362340)

Cui, Zhang, Du, Wang, Archacki, Zhang, Yuan, Ke, Li, Li, Li, Li, Tang, Yin, Liu: "HSF4 is involved in DNA damage repair through regulation of Rad51." dans: Biochimica et biophysica acta, Vol. 1822, Issue 8, pp. 1308-15, (2012) (PubMed).

Détails sur H2AFX

Antigène: H2AFX (H2A Histone Family, Member X (H2AFX))

Autre désignation: Histone H2A.X

Sujet: Variant histone H2A which replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Required for checkpoint-mediated arrest of cell cycle progression in response to low doses of ionizing radiation and for efficient repair of DNA double strand breaks (DSBs) specifically when modified by C-terminal phosphorylation.

Poids moléculaire: 15 kDa

NCBI Accession: NP_002096

UniProt: P16104

Pathways: Telomere Maintenance, Réparation de l'ADN, Positive Regulation of Response to DNA Damage Stimulus