HIF1A anticorps (AA 432-528)

N° du produit ABIN363317

L’anticorps anti-HIF1A Polyclonal Lapin est utilisé pour la détection de HIF1A dans des échantillons de Humain, Souris, Rat, Singe et Xenopus laevis. Il a été validé pour WB, IHC, IP, ICC, IF, IHC (p), ChIP et GS.

Aperçu rapide pour HIF1A anticorps (AA 432-528) (ABIN363317)

Antigène: HIF1A (Hypoxia Inducible Factor 1, alpha Subunit (Basic Helix-Loop-Helix Transcription Factor) (HIF1A))

Reactivité: Humain, Souris, Rat, Singe, Xenopus laevis

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp HIF1A est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Chromatin Immunoprecipitation (ChIP), Gel Shift (GS)

Détail du produit anti-HIF1A anticorps

Épitope: AA 432-528

Specificité: Reacts with HIF-1 alpha from human, monkey, mouse, rat and Xenopus

Réactivité croisée (Details): Mouse results are mixed.

Purification: Antigen affinity purified

Immunogène: Fusion protein containing amino acids 432-528 of human HIF-1 alpha.

Information d'application

Indications d'application:

Working dilution: Optimal dilution should be determined by the end user.
The following are guidelines only :
ChIP25 μL for 15 million cells, GS1:1-1:100, ICC/IF1:10-1:2000, IHC 1:10-1:2000, IHC-P1:100, IP1:1000, WB1:500-1:1000

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/mL

Buffer: Tris-glycine, 150 mM NaCl, Sodium azide 0.05 %

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C/-80 °C

Stockage commentaire: Aliquot and store at -20°C or -80°C. Avoid freeze-thaw cycles.

Détails sur HIF1A

Antigène: HIF1A (Hypoxia Inducible Factor 1, alpha Subunit (Basic Helix-Loop-Helix Transcription Factor) (HIF1A))

Autre désignation: Hypoxia-Inducible Factor 1-alpha

Sujet: Functions as a master transcriptional regulator of the adaptive response to hypoxia. Under hypoxic conditions, activates the transcription of over 40 genes, including erythropoietin, glucose transporters, glycolytic enzymes, vascular endothelial growth factor, HILPDA, and other genes whose protein products increase oxygen delivery or facilitate metabolic adaptation to hypoxia. Plays an essential role in embryonic vascularization, tumor angiogenesis and pathophysiology of ischemic disease. Binds to core DNA sequence 5'-[AG]CGTG-3' within the hypoxia response element (HRE) of target gene promoters. Activation requires recruitment of transcriptional coactivators such as CREBPB and EP300. Activity is enhanced by interaction with both, NCOA1 or NCOA2. Interaction with redox regulatory protein APEX seems to activate CTAD and potentiates activation by NCOA1 and CREBBP. Involved in the axonal distribution and transport of mitochondria in neurons during hypoxia.

ID gène: 3091

Pathways: Positive Regulation of Peptide Hormone Secretion, Regulation of Hormone Metabolic Process, Regulation of Hormone Biosynthetic Process, Cellular Response to Molecule of Bacterial Origin, Carbohydrate Homeostasis, Transition Metal Ion Homeostasis, Tube Formation, Regulation of Carbohydrate Metabolic Process, Signaling Events mediated by VEGFR1 and VEGFR2, VEGFR1 Specific Signals, L'effet Warburg