IL1R1 anticorps (Extracellular Domain)

N° du produit ABIN364253

Détail du produit anti-IL1R1 anticorps

Antigène: IL1R1 (Interleukin 1 Receptor, Type I (IL1R1))

Épitope: Extracellular Domain

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp IL1R1 est non-conjugé

Application: ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Radioimmunoassay (RIA)

Marque: IHC-plus™

Specificité: These antibodies react with cell surface and soluble human IL-1 receptor, Specific epitopes are unknown, but they do partially inhibit (30-50 % ) binding of one another to IL-1 receptor. They do not block binding of IL-1 to receptor, and they do not cross-react with receptors for human IL-2 or IL-4.

Purification: Protein G purified

Immunogène: The extracellular domain of the type I human IL-1 receptor.

Type of Immunogen: Protein

Clone: 40101

Isotype: IgG2b

Information d'application

Indications d'application: Approved: ELISA, IHC, IHC-P (10 μg/mL), RIA

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 10 μg/mL. ELISA: Can be used as capture reagents or second antibody at a concentration of 5 μg/mL. RIA: Concentrations of 20-30 ng/mL of these antibodies will bind radiolabeled IL-1 receptor in a radioimmunoassay format. Immunocytochemistry: IL-1 receptor can be detected on cultured cells using antibody concentrations of 10-100 μg/mL.

Not recommended for: WB

Commentaires:

Target Species of Antibody: Human

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.4. Sourced in Ascites.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: -20 °C,-80 °C

Stockage commentaire: Short term: -20°C
Long term: -70°C
Avoid freeze-thaw cycles.

Détails sur IL1R1

Antigène: IL1R1 (Interleukin 1 Receptor, Type I (IL1R1))

Autre désignation: IL1RA / IL1R1 (IL1R1 Produits)

Synonymes: anticorps CD121A, anticorps D2S1473, anticorps IL-1R-alpha, anticorps IL1R, anticorps IL1RA, anticorps P80, anticorps CD121a, anticorps CD121b, anticorps IL-iR, anticorps Il1r-1, anticorps DER4, anticorps Fit-1, anticorps Ly84, anticorps ST2L, anticorps St2, anticorps St2-rs1, anticorps T1, anticorps T1/ST2, anticorps interleukin 1 receptor type 1, anticorps interleukin 1 receptor, type I, anticorps interleukin 1 receptor-like 1, anticorps IL1R1, anticorps Il1r1, anticorps Il1rl1

Sujet: Name/Gene ID: IL1R1
Family: Interleukin

Synonyms: IL1R1, Antigen CD121a, D2S1473, IL-1R-1, IL-1R-alpha, IL-1RT-1, IL-1RT1, IL1R, IL1RA, IL1RT1, Interleukin receptor 1, Interleukin-1 receptor alpha, Interleukin-1 receptor type 1, CD121A, CD121a antigen, Interleukin 1 receptor, type I, Interleukin-1 receptor type I, Interleukin-1 type i receptor, p80

ID gène: 3554

UniProt: P14778

Pathways: Signalisation NF-kappaB, Carbohydrate Homeostasis, Cancer Immune Checkpoints