Syndecan 2 anticorps (Internal Region)

N° du produit ABIN364375

Détail du produit anti-Syndecan 2 anticorps

Antigène: Syndecan 2 (SDC2)

Épitope: Internal Region

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp Syndecan 2 est non-conjugé

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Marque: IHC-plus™

Specificité: Recognizes human syndecan-2 (fibroglycan). It recognizes the unglyconated form and may recognize the glyconated form. On Western Blots, it identifies a single band at ~22kD. An additional band at ~40kD may also be observed which may represent a glycanated form. Reactivity has been confirmed with human A-549 lung carcinoma, HEP-G2 hepatocellular carcinoma, HT-29 colorectal adenocarcinoma, K-562 chronic myelogenous leukemia-bone marrow, TF-1 erythroleukemia-bone marrow and U-2 OS osteosarcoma cell lysates.

Purification: Affinity purified

Immunogène: Synthetic peptide derived from the internal region of human syndecan-2 (fibroglycan).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Information d'application

Indications d'application: Approved: ELISA (0.1 - 1 μg/mL), IHC, IHC-P (5 μg/mL), WB (1 - 3 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Commentaires:

Target Species of Antibody: Human

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.4, 0.09 % sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C,-20 °C

Stockage commentaire: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Détails sur Syndecan 2

Antigène: Syndecan 2 (SDC2)

Autre désignation: SDC2 / Syndecan 2 (SDC2 Produits)

Synonymes: anticorps CD362, anticorps HSPG, anticorps HSPG1, anticorps SYND2, anticorps 4833414L08Rik, anticorps AA960457, anticorps Hspg1, anticorps Synd2, anticorps syndecan-2, anticorps cb440, anticorps zgc:103676, anticorps sdc2, anticorps SYND2-B, anticorps Xsyn-2, anticorps hspg, anticorps hspg1, anticorps sdc2-a, anticorps sdc2-b, anticorps synd2, anticorps syndecan 2, anticorps syndecan 2 L homeolog, anticorps syndecan 2 S homeolog, anticorps SDC2, anticorps Sdc2, anticorps sdc2, anticorps sdc2.L, anticorps sdc2.S

Sujet: Name/Gene ID: SDC2

Synonyms: SDC2, CD362, CD362 antigen, HSPG, HSPG1, Fibroglycan, Syndecan proteoglycan 2, SYND2, Syndecan 2, Syndecan-2

ID gène: 6383

UniProt: P34741

Pathways: Glycosaminoglycan Metabolic Process