Medium-Chain Specific Acyl-CoA Dehydrogenase, Mitochondrial (AA 441-454) anticorps

N° du produit ABIN364387

Détail du produit

Antigène: Medium-Chain Specific Acyl-CoA Dehydrogenase, Mitochondrial

Épitope: AA 441-454

Reactivité: Humain

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Inconjugué

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Marque: IHC-plus™

Specificité: Human ACADM. This antibody is expected to recognise both reported isoforms.

Homologie: Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla (100%) Gibbon, Marmoset, Mouse, Rat, Horse, Opossum (86%).

Purification: Immunoaffinity purified

Immunogène: Synthetic peptide C-RLIVAREHIDKYKN from the C-terminus of human ACADM (NP_000007.1, NP_001120800.1). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla (100%), Gibbon, Marmoset, Mouse, Rat, Horse, Opossum (86%).

Type of Immunogen: Synthetic peptide

Information d'application

Indications d'application: Approved: IHC, IHC-P (3.75 μg/mL), Peptide-ELISA (1:32000), WB (0.01 - 0.1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 3.75 μg/mL.

Commentaires:

Target Species of Antibody: Human

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Minimize freezing and thawing.

Stock: -20 °C

Stockage commentaire: Store at -20°C. Avoid freeze-thaw cycles.

Détail du antigène

Antigène: Medium-Chain Specific Acyl-CoA Dehydrogenase, Mitochondrial

Autre désignation: MCAD / ACADM

Synonymes: anticorps ACAD1, anticorps MCAD, anticorps MCADH, anticorps AU018656, anticorps acyl-CoA dehydrogenase medium chain, anticorps acyl-Coenzyme A dehydrogenase, medium chain, anticorps acyl-CoA dehydrogenase, C-4 to C-12 straight chain, anticorps ACADM, anticorps Acadm

Sujet: Name/Gene ID: ACADM

Synonyms: ACADM, ACAD1, MCAD, MCADH

ID gène: 34

NCBI Accession: NP_000007, NP_001120800

UniProt: P11310