GRB2 anticorps

N° du produit ABIN364459

Cet anticorps Souris Monoclonal détecte spécifiquement GRB2 dans WB, IHC et IHC (p). Il présente une réactivité envers Humain, Souris, Rat, Boeuf (Vache) et Hamster.

Aperçu rapide pour GRB2 anticorps (ABIN364459)

Antigène: GRB2 (Growth Factor Receptor-Bound Protein 2 (GRB2))

Reactivité: Humain, Souris, Rat, Boeuf (Vache), Hamster

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp GRB2 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Détail du produit anti-GRB2 anticorps

Marque: IHC-plus™

Specificité: Recognizes the N-terminal SH3 region of rat Grb2 at 23-25kD.

Purification: Protein A purified

Immunogène: Recombinant rat GRB2/Ash.

Type of Immunogen: Recombinant protein

Isotype: IgG1 kappa

Information d'application

Indications d'application: Approved: IHC, IHC-P (5 μg/mL), WB (0.5 - 2 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. Western Blot: 0.5-2 mg/mL. Detects GRB2 (23-25kD) in RIPA lysates of non-stimulated human A431 carcinoma cells. Previous lots detected GRB2 in RIPA lysates of mouse 3T3 fibroblasts, rat L6 myoblasts, Chinese hamster ovary cells and bovine brain extract.

Commentaires:

Target Species of Antibody: Rat

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: Lot specific

Buffer: PBS, 50 % glycerol.

Conseil sur la manipulation: Avoid repeat freeze-thaw cycles.

Stock: 4 °C,-20 °C

Stockage commentaire: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Détails sur GRB2

Antigène: GRB2 (Growth Factor Receptor-Bound Protein 2 (GRB2))

Autre désignation: GRB2

Sujet: Name/Gene ID: GRB2

Synonyms: GRB2, Abundant SRC homology, Adapter protein GRB2, ASH, EGFRBP-GRB2, MST084, MSTP084, Protein Ash, SH2/SH3 adapter GRB2, Grb3-3, HT027, NCKAP2

ID gène: 2885

UniProt: P62993

Pathways: Signalisation RTK, TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Regulation of Actin Filament Polymerization, Hepatitis C, Signaling Events mediated by VEGFR1 and VEGFR2, Signaling of Hepatocyte Growth Factor Receptor, EGFR Downregulation