CREB1 anticorps (pSer133)

N° du produit ABIN372604

Cet anticorps Lapin Polyclonal détecte spécifiquement CREB1 dans WB et IHC (fro). Il présente une réactivité envers Humain, Souris, Rat, Poisson zèbre (Danio rerio), Boeuf (Vache), Poulet, Mouton, Chien, Xenopus laevis et Non-Human Primate.

Aperçu rapide pour CREB1 anticorps (pSer133) (ABIN372604)

Antigène: CREB1 (cAMP Responsive Element Binding Protein 1 (CREB1))

Reactivité: Humain, Souris, Rat, Poisson zèbre (Danio rerio), Boeuf (Vache), Poulet, Mouton, Chien, Xenopus laevis, Non-Human Primate

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp CREB1 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro))

Détail du produit anti-CREB1 anticorps

Épitope: pSer133

Specificité: Specific for the ~45k CREB protein phosphorylated at Ser133. Immunolabeling is blocked by lambda-phosphatase treatment.

Réactivité croisée (Details): Species reactivity (expected):Bovine, Canine, Chicken, Human, Mouse, Sheep, Xenopus, Zebrafish and non-Human Primates.
Species reactivity (tested):Rat.

Purification: Sequential Chromatography on phospho- and dephosphopeptide affinity columns.

Immunogène: Phosphopeptide corresponding to amino acid residues surrounding the phospho-Ser133 of rat CREB.

Isotype: IgG

Information d'application

Indications d'application: Western Blot: 1/1000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: 10 mM HEPES ( pH 7.5), 150 mM NaCl, 100 μg/mL BSA and 50 % Glycerol.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: -20 °C

Stockage commentaire: Store the antibody undiluted (in aliquots) at-20 °C.

Détails sur CREB1

Antigène: CREB1 (cAMP Responsive Element Binding Protein 1 (CREB1))

Autre désignation: CREB1

Sujet: It is well known that the control of gene expression involves activation of protein kinase cascades that regulate transcription factors within the nucleus (Karin and Hunter, 1995). The cyclic AMP response element binding protein (CREB) is one of the best characterized stimulus-induced transcription factors (Montminy, 1997). This transcription factor is a component of intracellular signaling events that regulate a wide range of biological functions, from spermatogenesis to circadian rhythms and memory (Shaywitz and Greenberg, 1999, Silva et al., 1998). A variety of protein kinases including protein kinase A (PKA), mitogen-activated protein kinases (MAPKs), and Ca2+/calmodulin-dependent protein kinases (CaMKs) phosphorylate CREB at serine 133 (Ser133), and phosphorylation of Ser133 are required for CREB-mediated transcription (Johannessen et al., 2004, Kornhauser et al., 2002).Synonyms: CREB-1, Cyclic AMP-responsive element-binding protein 1, cAMP-responsive element-binding protein 1

ID gène: 81646

NCBI Accession: NP_112279

UniProt: P15337

Pathways: Signalisation TLR, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Thyroid Hormone Synthesis, Activation of Innate immune Response, Myometrial Relaxation and Contraction, Regulation of Cell Size, Toll-Like Receptors Cascades, G-protein mediated Events, Interaction of EGFR with phospholipase C-gamma, Positive Regulation of fat Cell Differentiation