MEK1 anticorps (pThr292)

N° du produit ABIN372665

L’anticorps anti-MEK1 Polyclonal Lapin est utilisé pour la détection de MEK1 dans des échantillons de Humain, Souris, Rat, Chien, Boeuf (Vache) et Non-Human Primate. Il a été validé pour WB.

Aperçu rapide pour MEK1 anticorps (pThr292) (ABIN372665)

Antigène: MEK1 (MAP2K1) (Mitogen-Activated Protein Kinase Kinase 1 (MAP2K1))

Reactivité: Humain, Souris, Rat, Chien, Boeuf (Vache), Non-Human Primate

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp MEK1 est non-conjugé

Application: Western Blotting (WB)

Détail du produit anti-MEK1 anticorps

Épitope: pThr292

Specificité: This antibody is specific for the ~45k MEK 1 protein phosphorylated at Thr292 in Western blots.

Réactivité croisée (Details): Species reactivity (expected):Mouse, Human, Bovine, Canine and non-Human Primates.
Species reactivity (tested):Rat.

Purification: Sequential Chromatography on phospho- and dephosphopeptide affinity columns.

Immunogène: Phosphopeptide corresponding to amino acid residues surrounding the phospho-Thr292 of human MEK1.

Isotype: IgG

Information d'application

Indications d'application: Western blot: 1/1000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: 10 mM HEPES ( pH 7.5), 150 mM NaCl, 100 μg/mL BSA and 50 % Glycerol.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: -20 °C

Stockage commentaire: Store the antibody undiluted (in aliquots) at-20 °C.

Détails sur MEK1

Antigène: MEK1 (MAP2K1) (Mitogen-Activated Protein Kinase Kinase 1 (MAP2K1))

Autre désignation: MAP2K1

Sujet: MEK1 (MAP Kinase Kinase, also known as MKK) is an integral component of the MAP kinase cascade that regulates cell growth and differentiation (Ahn, 1993, Chong et al., 2003). This pathway also plays a key role in synaptic plasticity in the brain (Adams and Sweatt, 2002). Activated MEK 1 acts as a dual specificity kinase phosphorylating both a threonine and a tyrosine residue on MAP kinase (Kyriakis et al., 1991, Seger et al., 1991, Crews et al., 1992). Conversely, there also appears to be a feedback phosphorylation of MEK 1 by MAP kinase. The sites on MEK 1 that are phosphorylated by MAP kinase are Thr292 and Thr386 (Mansour et al., 1994).Synonyms: Dual specificity mitogen-activated protein kinase kinase 1, ERK activator kinase 1, MAP kinase kinase 1, MAPK/ERK kinase 1, MAPKK 1, MEK1, PRKMK1

ID gène: 5604

NCBI Accession: NP_002746

UniProt: Q02750

Pathways: Signalisation MAPK, Signalisation RTK, Interferon-gamma Pathway, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Toll-Like Receptors Cascades, Autophagy, Signaling of Hepatocyte Growth Factor Receptor, BCR Signaling