MEK1 anticorps (pThr386)

N° du produit ABIN372666

Détail du produit anti-MEK1 anticorps

Antigène: MEK1 (MAP2K1) (Mitogen-Activated Protein Kinase Kinase 1 (MAP2K1))

Épitope: pThr386

Reactivité: Humain, Souris, Rat, Chien, Boeuf (Vache), Poulet, Xenopus laevis, Non-Human Primate

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp MEK1 est non-conjugé

Application: Western Blotting (WB)

Specificité: This antibody is specific for the ~45k MEK 1 protein phosphorylated at Thr386. The immunolabeling is completely eliminated by treatment with lambda-phosphatase.

Réactivité croisée (Details): Species reactivity (expected):Mouse, Human, Chicken, Bovine, Canine, non-Human Primates and Xenopus.
Species reactivity (tested):Rat.

Purification: Sequential Chromatography on phospho- and dephosphopeptide affinity columns.

Immunogène: Phosphopeptide corresponding to amino acid residues surrounding the phospho-Thr386 of human MEK 1.

Isotype: IgG

Information d'application

Indications d'application: Western blot: 1/1000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: 10 mM HEPES ( pH 7.5), 150 mM NaCl, 100 μg/mL BSA and 50 % Glycerol.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: -20 °C

Stockage commentaire: Store the antibody undiluted (in aliquots) at-20 °C.

Détails sur MEK1

Antigène: MEK1 (MAP2K1) (Mitogen-Activated Protein Kinase Kinase 1 (MAP2K1))

Autre désignation: MAP2K1 (MAP2K1 Produits)

Synonymes: anticorps Mek1, anticorps CFC3, anticorps MAPKK1, anticorps MEK1, anticorps MKK1, anticorps PRKMK1, anticorps MEKK1, anticorps Prkmk1, anticorps mek-2, anticorps si:ch211-242m18.2, anticorps wu:fj56a12, anticorps wu:fj61b01, anticorps zgc:56557, anticorps ATMEK1, anticorps F20B18.180, anticorps F20B18_180, anticorps MAP kinase/ ERK kinase 1, anticorps MITOGEN ACTIVATED PROTEIN KINASE KINASE, anticorps MITOGEN ACTIVATED PROTEIN KINASE KINASE 1, anticorps NMAPKK, anticorps MEK, anticorps mitogen activated protein kinase kinase 1, anticorps mitogen-activated protein kinase kinase 1, anticorps mitogen-activated protein kinase kinase 1 L homeolog, anticorps MAP kinase/ ERK kinase 1, anticorps Map2k1, anticorps MAP2K1, anticorps map2k1.L, anticorps map2k1, anticorps MEK1

Sujet: MEK1 (MAP Kinase Kinase, also known as MKK) is an integral component of the MAP kinase cascade that regulates cell growth and differentiation (Ahn, 1993, Chong et al., 2003). This pathway also plays a key role in synaptic plasticity in the brain (Adams and Sweatt, 2002). Activated MEK 1 acts as a dual specificity kinase phosphorylating both a threonine and a tyrosine residue on MAP kinase (Kyriakis et al., 1991, Seger et al., 1991, Crews et al., 1992). Conversely, there also appears to be a feedback phosphorylation of MEK 1 by MAP kinase. The sites on MEK 1 that are phosphorylated by MAP kinase are Thr292 and Thr386 (Mansour et al., 1994).Synonyms: Dual specificity mitogen-activated protein kinase kinase 1, ERK activator kinase 1, MAP kinase kinase 1, MAPK/ERK kinase 1, MAPKK 1, MEK1, PRKMK1

ID gène: 5604

NCBI Accession: NP_002746

UniProt: Q02750

Pathways: Signalisation MAPK, Signalisation RTK, Interferon-gamma Pathway, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Toll-Like Receptors Cascades, Autophagy, Signaling of Hepatocyte Growth Factor Receptor, BCR Signaling