DAZL anticorps (Internal Region)
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- Antigène Voir toutes DAZL Anticorps
- DAZL (Deleted in Azoospermia-Like (DAZL))
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Épitope
- Internal Region
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Reactivité
- Humain, Souris, Rat, Boeuf (Vache), Chien
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Hôte
- Chèvre
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Clonalité
- Polyclonal
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Conjugué
- Cet anticorp DAZL est non-conjugé
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Application
- Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)
- Séquence
- C-GNGPQKKSVD R
- Specificité
- This antibody recognizes DAZL.
- Réactivité croisée (Details)
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Species reactivity (expected):Rat, Canine, Bovine.
Species reactivity (tested):Human, Mouse. - Purification
- Ammonium sulphate precipitation followed by antigen Affinity Chromatography using the immunizing peptide.
- Immunogène
- Synthetic peptide corresponding to the internal sequence of Human DAZL according to NP_001342.2 Genename: DAZL
- Top Product
- Discover our top product DAZL Anticorps primaire
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- Indications d'application
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Peptide ELISA: 1/64000. Western blot: Approx 35 kDa band observed in human ovary and human testes lysates(calculated MW of 33.5 kDa according to NP_001342.2). Recommended concentration: 0.1 - 0.3 μg/mL. Immunohistochemistry on paraffin sections: Mouse Testis shows cytoplasm staining ofspermatogonia. Additional staining is observed in the cytoplasm of elongatng spermatidsbut this additional staining is not in agreement with results obtained by another antibody(Ruggiuet al, Nature.1997 Sep 4,389(6646): 73-7. PMID: 9288969). Data kindly provided by J. S. Martins, MRC, Edinburgh, UK. Recommended concentration: 1 - 3 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - Restrictions
- For Research Use only
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- Concentration
- 0.5 mg/mL
- Buffer
- Tris saline, pH 7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer
- Agent conservateur
- Sodium azide
- Précaution d'utilisation
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Conseil sur la manipulation
- Avoid repeated freezing and thawing.
- Stock
- 4 °C/-20 °C
- Stockage commentaire
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C.
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- Antigène
- DAZL (Deleted in Azoospermia-Like (DAZL))
- Autre désignation
- DAZL (DAZL Produits)
- Synonymes
- anticorps DAZH, anticorps DAZL1, anticorps DAZLA, anticorps SPGYLA, anticorps DAZL, anticorps Xdazl, anticorps dazh, anticorps dazl1, anticorps dazla, anticorps spgyla, anticorps dazl-B, anticorps dazl-a, anticorps Daz-like, anticorps Dazh, anticorps Dazl1, anticorps Dazla, anticorps Tpx-2, anticorps Tpx2, anticorps deleted in azoospermia like, anticorps deleted in azoospermia-like, anticorps deleted in azoospermia-like L homeolog, anticorps DAZL, anticorps dazl, anticorps Dazl, anticorps dazl.L
- Sujet
- DAZL is a member of the DAZ (deleted in azoospermia) family of RNA binding proteins. DAZL is expressed in fetal and adult testes and ovaries, and is believed to play a role in germ cell development. In adult germ cells, the expression of DAZL is localised to the cytoplasm. Mutations in this gene have been linked to severe spermatogenic failure and infertility in males.Synonyms: DAZ homolog, DAZ-like autosomal, DAZH, DAZL, DAZL1, DAZLA, Deleted in azoospermia-like, Deleted in azoospermia-like 1, SPGY-like-autosomal, SPGYLA
- ID gène
- 1618
- NCBI Accession
- NP_001342
- UniProt
- Q92904
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