Arylsulfatase B anticorps (Internal Region)

N° du produit ABIN374887

Détail du produit anti-Arylsulfatase B anticorps

Antigène: Arylsulfatase B (ARSB)

Épitope: Internal Region

Reactivité: Humain

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp Arylsulfatase B est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Séquence: C-KLARGHTNGT KPLD

Specificité: This antibody detects internal Arylsulfatase B. It is expected to recognise both reported isoforms (NP_000037.2, NP_942002.1).

Réactivité croisée (Details): Species reactivity (tested):Human

Purification: Ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide

Immunogène: Peptide from the internal region of the protein sequence according to NP_000037.2, NP_942002.1.

Information d'application

Indications d'application: Peptide ELISA: limit dilution 1: 64000. Western blot: 0.03-0.1 μg/mL. Approx 45 kDa band observed in Human Heart lysates(calculated MW of 46.5 kDa according to NP_942002.1). An additional band of unknownidentity was also consistently observed at 35 kDa. This band was successfully blocked byincubation with the immunising peptide. We would appreciate any feedback from peoplein the field - have any such results been reported with other antibodies/lysates? Have anyfurther splice variants/modified forms been reported?Immunohistochemistryon paraffin sections: 5-10 μg/mL. In Human Skeletal Muscle showsstriated pattern staining of majority of muscular fibres.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 0.5 mg/mL

Buffer: Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer.

Références pour anticorps anti-Arylsulfatase B (ABIN374887)

Zhao, Wang, Li, Dong, Wu: "Arylsulfatase B Mediates the Sulfonation-Transport Interplay in Human Embryonic Kidney 293 Cells Overexpressing Sulfotransferase 1A3." dans: Drug metabolism and disposition: the biological fate of chemicals, Vol. 44, Issue 9, pp. 1441-9, (2018) (PubMed).

Détails sur Arylsulfatase B

Antigène: Arylsulfatase B (ARSB)

Autre désignation: Arylsulfatase B (ARSB Produits)

Synonymes: anticorps ASB, anticorps G4S, anticorps MPS6, anticorps 1110007C02Rik, anticorps AI480648, anticorps As-1, anticorps As-1r, anticorps As-1s, anticorps As-1t, anticorps As1, anticorps As1-r, anticorps As1-s, anticorps As1-t, anticorps Asr-1, anticorps Ast-1, anticorps ARSB, anticorps arylsulfatase B, anticorps arylsulfatase, anticorps arylsulfatase b, anticorps ARSB, anticorps Arsb, anticorps RB348, anticorps LOC5566067, anticorps CpipJ_CPIJ011047, anticorps VDBG_03275, anticorps arsb, anticorps LOC5579667

Sujet: Arylsulfatase B (ARSB) belongs to the sulfatase family. The arylsulfatase B homodimer catalyzes hydrolysis of the sulfate groups of N-Acetyl-D-galactosamine, chondriotin sulfate, and dermatan sulfate. Defects in this gene cause Maroteaux-Lamy syndrome.Synonyms: ARSB, ASB, G4S, N-acetylgalactosamine-4-sulfatase

ID gène: 411, 9606

UniProt: P15848

Pathways: Glycosaminoglycan Metabolic Process