Peptide ELISA: 1/8000. Western Blot: 0.1 - 0.3 μg/mL. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions
For Research Use only
Concentration
0,5 mg/mL
Buffer
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin
Agent conservateur
Sodium azide
Précaution d'utilisation
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Conseil sur la manipulation
Avoid repeated freezing and thawing.
Stock
4 °C/-20 °C
Stockage commentaire
Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
Antigène
NDEL1
(NudE Nuclear Distribution E Homolog (A. Nidulans)-Like 1 (NDEL1))
anticorps EOPA, anticorps MITAP1, anticorps NDE1L1, anticorps NDE2, anticorps NUDEL, anticorps NUDE2, anticorps Nudel, anticorps 2600006O07Rik, anticorps mNudel, anticorps wu:fi23c04, anticorps wu:fi23e06, anticorps eopa, anticorps mitap1, anticorps ndel1, anticorps ndel1-a, anticorps ndel1-b, anticorps nudel, anticorps nudE neurodevelopment protein 1 like 1, anticorps nudE neurodevelopment protein 1-like 1, anticorps nudE neurodevelopment protein 1-like 1b, anticorps nudE neurodevelopment protein 1 like 1 L homeolog, anticorps NDEL1, anticorps Ndel1, anticorps ndel1b, anticorps ndel1.L
Sujet
Nudel is important for normal cortical development. It is invovled in microtubule organization, nuclear translocation, and neuronal positioning in concert with various other factors (including Lis1, Pafah1b1, Pahfah1b2, dynein, dynorphin A and cdk5). Western blot analysis of mouse tissues shows abundant expression of Nudel in brain and testis, and much lower expression in heart, liver, kidney, and skeletal muscle. In fractionated rat brain, Nudel and Lis1 are both found in fractions enriched for postsynaptic density proteins. Immunostaining of embryonic day 18 mouse brain sections revealed staining of migrating neurons and thalamocortical axons of the intermediate zone of the developing cerebral cortex, as well as several other developing brain regions. The deduced protein contains 345 amino acids and has a calculated molecular mass of 38.4 kDa. It has a coiled coil motif (residues 19 to 201), followed by several potential phosphorylation sites for casein kinase II, protein kinase C or CDK5. Nudel shares about 50 % identity with mouse and human NUDE proteins.Synonyms: EOPA, MITAP1, NUDEL, Nuclear distribution protein nudE-like 1