CENPF anticorps (AA 1759-2093)

N° du produit ABIN396951

Cet anticorps anti-CENPF est un anticorps Souris Monoclonal détectant CENPF dans IHC, WB, IP, IHC (p) et IHC (fro). Adapté pour Humain.

Aperçu rapide pour CENPF anticorps (AA 1759-2093) (ABIN396951)

Antigène: CENPF (Centromere Protein F (CENPF))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp CENPF est non-conjugé

Application: Immunohistochemistry (IHC), Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Détail du produit anti-CENPF anticorps

Épitope: AA 1759-2093

Marque: IHC-plus™

Specificité: Recognizes mitosin, a 350 kD phosphoprotein that is specifically involved in mitotic-phase progression. Mitosin is expressed throughout S, G2, and M phases of the cell cycle, but is absent in G0 and G1. It associates with the mitotic apparatus during M-phase and is redistributed from the nucleus to the centromere, spindle, and midbody during M-phase progression. Its usefulness as a proliferation marker is under active investigation.

Purification: Protein G purified

Immunogène: GST fusion protein expressed in E. coli corresponding to aa 1759-2093 of human mitosin.

Type of Immunogen: Fusion protein

Isotype: IgG2a kappa

Information d'application

Indications d'application: Approved: IHC, IHC-Fr (10 μg/mL), IHC-P (10 μg/mL), IP (1 - 10 μg/mL), WB (1 - 10 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 10 μg/mL. Western Blot: 1-10 μg/mL. Immunoprecipitation: 1-10 μg/mL. Immunohistochemistry: 10 μg/mL with frozen or paraffin-embedded sections. Tested in IHC using formalin-fixed paraffin-embedded human tissues, HIER antigen retrieval using sodium citrate buffer at  pH 6.0.

Commentaires:

Target Species of Antibody: Human

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.4

Conseil sur la manipulation: Avoid freeze/thaw cycles.

Stock: -80 °C

Stockage commentaire: Store at -70°C, avoid freeze/thaw cycles.

Détails sur CENPF

Antigène: CENPF (Centromere Protein F (CENPF))

Autre désignation: CENPF / CENP-F

Sujet: Name/Gene ID: CENPF

Synonyms: CENPF, AH antigen, Centromere protein F, CENF, CENP-F, Kinetochore protein CENPF, Mitosin, CENP-F kinetochore protein, Hcp-1, PRO1779

ID gène: 1063

UniProt: P49454

Pathways: Chromatin Binding, M Phase, SARS-CoV-2 Protein Interactome, Phosphorylation & l'infection par le SRAS-CoV-2