GST anticorps (HRP)

N° du produit ABIN398845

Détail du produit anti-GST anticorps (HRP)

Antigène: GST (Glutathione S Transferase (GST))

Reactivité: Schistosoma japonicum

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp GST est conjugé à/à la HRP

Application: Western Blotting (WB), ELISA

Specificité: Goat Anti-GST-tag [HRP] Polyclonal Antibody detects GST fusion proteins in expression systems including bacterial expression system, mammalian expression system and others.

Purification: Immunoaffinity chromatography

Immunogène: Recombinant Glutathione S-Transferase (GST) from Schistosoma japonicum.

Isotype: IgG

Information d'application

Indications d'application: Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.

ELISA: 0.05-0.2 µg/mL Western blot: 0.2-1 µg/mLOther Applications: user-optimized

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Reconstitute the lyophilized powder with deionized water (or equivalent) to a final concentration of 0.5 mg/mL.

Buffer: PBS, pH 7.4, containing 1 % BSA and 0.01 % thimerosal.

Agent conservateur: Thimerosal (Merthiolate)

Précaution d'utilisation: This product contains thimerosal (merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C/-20 °C

Stockage commentaire: The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.

Références pour anticorps anti-GST (HRP) (ABIN398845)

Savar, Jahanian-Najafabadi, Mahdavi, Shokrgozar, Jafari, Bouzari et al.: "In silico and in vivo studies of truncated forms of flagellin (FliC) of enteroaggregative Escherichia coli fused to FimH from uropathogenic Escherichia coli as a vaccine candidate against urinary ..." dans: Journal of biotechnology, Vol. 175, pp. 31-7, (2014) (PubMed).

Gish, Gagne, Han, Deyoung, Clark: "WUSCHEL-responsive At5g65480 interacts with CLAVATA components in vitro and in transient expression." dans: PLoS ONE, Vol. 8, Issue 6, pp. e66345, (2013) (PubMed).

Détails sur GST

Antigène: GST (Glutathione S Transferase (GST))

Autre désignation: Glutathione-S-Transferase (GST) (GST Produits)

Synonymes: anticorps GST, anticorps GST 13-13, anticorps GST13, anticorps GST13-13, anticorps GSTK1-1, anticorps hGSTK1, anticorps 1500002K10Rik, anticorps Gst, anticorps Ptgds2, anticorps glutathione S-transferase kappa 1, anticorps microsomal glutathione S-transferase 1, anticorps hematopoietic prostaglandin D synthase, anticorps GSTK1, anticorps Mgst1, anticorps Hpgds

Sujet: Schistosoma japonicum glutathione S-transferase (GST) is a 26,000 Da enzyme that conjugates reduced glutathione to hydrophobic electrophiles. GST is an ideal fusion partner for production of foreign proteins in commercially available expression systems because it can be expressed in high levels in E. coli. The fusion proteins can be purified to homogeneity in a single step as the GST portion of the protein binds tightly to immobilized glutathione. Elution can be accomplished by using free glutathione. The GST portion of the fusion protein can be separated from the protein of interest by introducing site-specific proteases. Antibodies directed against GST can detect both the fusion protein during purification and the cleavage of GST from the protein of interest.Goat Anti-GST-tag [HRP] Polyclonal Antibody is developed in goat using a recombinant Glutathione S-Transferase (GST) from Schistosoma japonicum. The antibody is highly purified by immunoaffinity chromatography from goat antiserum and is then conjugated to horseradish peroxidase.