NRF1 anticorps (N-Term)
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- Antigène Voir toutes NRF1 Anticorps
- NRF1 (Nuclear Respiratory Factor 1 (NRF1))
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Épitope
- AA 1-534, N-Term
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Reactivité
- Humain, Souris, Rat, Poisson zèbre (Danio rerio), Poulet, Chimpanzé, Grenouille
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Hôte
- Lapin
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Clonalité
- Polyclonal
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Conjugué
- Cet anticorp NRF1 est non-conjugé
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Application
- Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)
- Réactivité croisée (Details)
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Species reactivity (expected):Human, Chimpanzee, Canine, Rat, Chicken, Frog, Zebrafish.
Species reactivity (tested):Mouse. - Purification
- Affinity Chromatography on Protein A.
- Immunogène
- Recombinant Mouse NRF1 protein corresponding to aa 1-534 of the native protein. Genename: NRF1
- Isotype
- IgG
- Top Product
- Discover our top product NRF1 Anticorps primaire
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- Indications d'application
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ELISA: 1/3000-1/10000. Western Blot: 1/500-1/2000. Immunohistochemistry on Paraffin Sections: 2 mg/mL-5 μg/mL. Expect a band approx. 67 kDa in size corresponding to NRF1 by Western blotting in theappropriate cell lysate or extract. Splice variants exist for this protein that may result in the detection of Lower MolecularWeight bands.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - Restrictions
- For Research Use only
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- Concentration
- 2.3 mg/mL (by UV absorbance at 280 nm)
- Buffer
- 0.02 M Potassium Phosphate, 0.12 M Sodium Chloride, pH 7.2 containing 0.01 % (w/v) Sodium Azide as preservative.
- Agent conservateur
- Sodium azide
- Précaution d'utilisation
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Conseil sur la manipulation
- Avoid repeated freezing and thawing.
- Stock
- -20 °C
- Stockage commentaire
- Store the antibody (in aliquots) at-20 °C. Dilute only prior to immediate use.
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- Antigène
- NRF1 (Nuclear Respiratory Factor 1 (NRF1))
- Autre désignation
- NRF1 (NRF1 Produits)
- Synonymes
- anticorps ALPHA-PAL, anticorps C87038, anticorps D6Ertd415e, anticorps SC:BZ3C13.1, anticorps SC:bZ3C13.2, anticorps ube2h, anticorps Nfe2l1_retired, anticorps IBR/F, anticorps NRF1, anticorps alpha-pal, anticorps nrf-1, anticorps LOC100220087, anticorps nuclear respiratory factor 1, anticorps nuclear respiratory factor 1 S homeolog, anticorps NRF1, anticorps Nrf1, anticorps nrf1, anticorps nrf1.S
- Sujet
- NRF1 (also known as nuclear respiratory factor 1, alpha palindromic binding protein and alpha-pal) is the mammalian homolog to the erect wing (ewg) Drosophila protein that is required for proper development of the central nervous system and indirect flight muscles. In mammals NRF1 functions as a transcription factor that activates the expression of the EIF2S1 (EIF2-alpha) gene. This protein links the transcriptional modulation of key metabolic genes to cellular growth and development and has been implicated in the control of nuclear genes required for respiration, heme biosynthesis, and mitochondrial DNA transcription and replication. NRF1 forms a homodimer and binds DNA as a dimer. NRF1 shows a nuclear localization and is widely expressed in embryonic, fetal, and adult tissues. Phosphorylation of NRF1 enhances DNA binding. Multiple splice variants have been identified for this protein.Synonyms: Alpha palindromic-binding protein, Alpha-pal, NRF-1, Nuclear respiratory factor 1
- ID gène
- 10090
- UniProt
- Q9WU00
- Pathways
- Regulation of Lipid Metabolism by PPARalpha
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