OCT4 anticorps (Internal Region)
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- Antigène Voir toutes OCT4 (POU5F1) Anticorps
- OCT4 (POU5F1) (POU Class 5 Homeobox 1 (POU5F1))
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Épitope
- Internal Region
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Reactivité
- Humain, Souris, Cheval, Chimpanzé, Macaque
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Hôte
- Lapin
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Clonalité
- Polyclonal
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Conjugué
- Cet anticorp OCT4 est non-conjugé
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Application
- Western Blotting (WB), Enzyme Immunoassay (EIA)
- Specificité
- This antibody reacts with Oct-4 protein.
- Réactivité croisée (Details)
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Species reactivity (expected):Chimpanzee, Macaque, Horse, Mink, Canine, Bovine, Porcine.
Species reactivity (tested):Human, Mouse. - Purification
- Affinity chromatography
- Immunogène
- Synthetic peptide corresponding to an internal region of human Oct-4 protein
- Isotype
- IgG
- Top Product
- Discover our top product POU5F1 Anticorps primaire
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- Indications d'application
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ELISA: 1/350,000. Western blot: 1/1,000 - 1/1,500.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - Restrictions
- For Research Use only
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- Concentration
- 1.0 mg/mL (by UV absorbance at 280 nm)
- Buffer
- 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 containing 0.01 % (w/v) Sodium Azide
- Agent conservateur
- Sodium azide
- Précaution d'utilisation
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Conseil sur la manipulation
- Avoid repeated freezing and thawing.
- Stock
- -20 °C
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- Antigène
- OCT4 (POU5F1) (POU Class 5 Homeobox 1 (POU5F1))
- Autre désignation
- OCT3/4 (POU5F1 Produits)
- Synonymes
- anticorps OCT3, anticorps OCT4, anticorps OTF-3, anticorps OTF3, anticorps OTF4, anticorps Oct-3, anticorps Oct-4, anticorps POU5F1, anticorps gp9, anticorps gp-9, anticorps oct4, anticorps pou2, anticorps POU-2, anticorps cb197, anticorps spg/pou2, anticorps chunp6868, anticorps wu:fd18d06, anticorps NF-A3, anticorps Oct-3/4, anticorps Oct3, anticorps Oct3/4, anticorps Oct4, anticorps Otf-3, anticorps Otf-4, anticorps Otf3, anticorps Otf3-rs7, anticorps Otf3g, anticorps Otf4, anticorps POU class 5 homeobox 1, anticorps POU domain, class 5, transcription factor 3, anticorps POU domain, class 5, transcription factor 1, anticorps POU5F1, anticorps pou5f3, anticorps Pou5f1
- Sujet
- Oct-4, also known as POU domain transcription factor, Octomer- binding transcription factor 3 and Oct-3, is expressed in human and mouse totipotent embryonic stem and germ cells. It may function in preventing these cells from differentiating. Each type of established mammalian pluripotent stem cell line (embryonal carcinoma (EC) cells, embryonic stem (ES) cells and embryonic germ (EG) cells) expresses Oct-4, which disappears rapidly when the cells differentiate. Most studies on Oct-4 have been performed on murine embryos. In human oocytes of various maturity and normally developing preimplantation/cleavage embryos, a variable Oct-4 expression pattern has been revealed, concomitant with a pure cytoplasmic localization of the protein. This variability in expression fades away during compaction, indicating embryonic Oct-4 expression, the protein appears in the nucleus, implying biological activity. Functional Oct-4 has been found in human amniotic fluid. As Oct-4 transcripts have also been found in various differentiated cells, the presence of Oct-4 transcripts or proteins may not be sufficient for identifying undifferentiated cell lines in humans. It may also be important to examine the localization of Oct-4 proteins within a cell. Oct-4 has been shown to interact with Nanog and with proteins from multiple repression complexes, including the NuRD, Sin3A and Pml complexes, possibly to control ES fate. Reactivation of Oct-4 expression is postulated to occur in differentiated cells that have undergone carcinogenesis, or tumor formation.Synonyms: OCT3, OCT4, OTF3, Oct-3, Oct-4, Octamer-binding transcription factor 3, POU domain, POU5F1, class 5, transcription factor 1
- ID gène
- 5460
- NCBI Accession
- NP_002692
- UniProt
- Q01860
- Pathways
- Stem Cell Maintenance
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