HMGA1 anticorps (Internal Region)

N° du produit ABIN401518

Détail du produit anti-HMGA1 anticorps

Antigène: HMGA1 (High Mobility Group AT-Hook 1 (HMGA1))

Épitope: AA 9-21, Internal Region

Reactivité: Humain, Souris, Rat, Chien, Porc, Boeuf (Vache)

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp HMGA1 est non-conjugé

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Séquence: c-SQPLASKQEK DGT

Specificité: This antibody is expected to recognize reported isoforms apha (NP_665906.1, NP_665908.1, NP_665911.1) and beta (NP_002122.1, NP_665910.1, NP_665912.1). This antibody is not expected to cross-react with HMGA2.

Réactivité croisée (Details): Species reactivity (expected):Mouse, Rat, Canine (Dog), Bovine, Pig.
Species reactivity (tested):Human.

Purification: Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide.

Immunogène: Peptide from the internal region (near N Terminus) of the protein sequence according to NP_665906.1, NP_002122.1. Genename: HMGA1

Information d'application

Indications d'application: Peptide ELISA: Detection Limit: 1/16000. Western blot: 0.5-1 μg/mL. Approx 24 kDa band observed in Human Pancreas (calculatedMW of 11.4 kDa/10.4 kDa according to NP_665906.1, NP_002122.1). The observed molecularweight corresponds to earlier findings with different antibodies from other commercialsources. This band was successfully blocked by incubation with the immunizing peptide.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH 7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

Détails sur HMGA1

Antigène: HMGA1 (High Mobility Group AT-Hook 1 (HMGA1))

Autre désignation: HMGA1 / HMGIY (HMGA1 Produits)

Synonymes: anticorps HMG-R, anticorps HMGA1A, anticorps HMGIY, anticorps Hmgi, anticorps Hmgiy, anticorps hmga1, anticorps hmgiy, anticorps wu:fb12b06, anticorps wu:fb18f11, anticorps wu:fc19f01, anticorps wu:fi38b05, anticorps zgc:85677, anticorps AL023995, anticorps Hmga1a, anticorps Hmga1b, anticorps Hmgy, anticorps HMG-I(Y), anticorps HMG-Y, anticorps HMGA1, anticorps zgc:153948, anticorps high mobility group AT-hook 1, anticorps high mobility group protein HMG-I/HMG-Y, anticorps high mobility group AT-hook 1a, anticorps high mobility group AT-hook 1b, anticorps HMGA1, anticorps Hmga1, anticorps TSC_c24220, anticorps hmga1a, anticorps LOC705105, anticorps hmga1b

Sujet: HMGA1 (HMGI/Y) is a non-histone protein involved in many cellular processes, including regulation of inducible gene transcription, integration of retroviruses into chromosomes, and the metastatic progression of cancer cells. There are two splice isoforms of HMGA1 - the full length HMGA1a/HMGI and the shortened form HMGA1b/HMGY. The encoded protein preferentially binds to the minor groove of A+T-rich regions in double-stranded DNA. It has little secondary structure in solution but assumes distinct conformations when bound to substrates such as DNA or other proteins. HMGA1 is frequently acetylated and is found in the nucleus. Interacts with HIV-1 pre-integration complex.Synonyms: High mobility group AT-hook protein 1, High mobility group protein A1, High mobility group protein HMG-I/HMG-Y, High mobility group protein R

ID gène: 3159

NCBI Accession: NP_002122

UniProt: P17096

Pathways: Nuclear Hormone Receptor Binding, Autophagy