CD3 epsilon anticorps (Activation Site)

N° du produit ABIN457415

L’anticorps Souris Monoclonal anti-CD3 epsilon a été validé pour FACS, WB, IP, IHC (fro) et ICC. Il convient pour détecter CD3 epsilon dans des échantillons de Humain et Souris. Il y a 7+ publications disponibles.

Aperçu rapide pour CD3 epsilon anticorps (Activation Site) (ABIN457415)

Antigène: CD3 epsilon (CD3E)

Reactivité: Humain, Souris

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp CD3 epsilon est non-conjugé

Application: Flow Cytometry (FACS), Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunocytochemistry (ICC)

Clone: APA1-1

Détail du produit anti-CD3 epsilon anticorps

Épitope: Activation Site

Fonction: Anti-CD3 epsilon (activation epitope) Purified

Specificité: The mouse monoclonal antibody APA1/1 recognizes an activation-dependent intracellular epitope of CD3 epsilon. Exposure of the epitope precedes CD3 phosphorylation and recruitment and activation of ZAP70, which initiates the signaling cascade produced by T-cell activation. APA1/1 provides the earliest known marker for TCR-mediated T cell activation.

Réactivité croisée (Details): Human, Mouse

Purification: Purified by protein-A affinity chromatography.

Pureté: > 95 % (by SDS-PAGE)

Immunogène: Purified human CD3 proteins isolated from thymus

Isotype: IgG1

Information d'application

Indications d'application: Flow cytometry: Intracellular staining, recommended dilution: 1-4 μg/mL, positive control: human T cells stimulated with anti-CD3 (MEM-57) antibody (1 μg/mL). Sample preparation: At the end of stimulation of T cells, perform staining of surface markers (if required) in PBS + 0.1 % BSA for 20 min. on ice. Wash with PBS and fix with 2 % formaldehyde, 30 min on ice. Wash with PBS and incubate in PBS + 0,1 % saponine, 5 min. RT. Incubate the cells in PBS + 1 % BSA + 0.03 % saponine, 15 min. on ice. Incubate with fluorescence-labeled APA1/1 antibody (1-2 μg/mL) in PBS + 1 % BSA + 0.03 % saponine in dark, 20 min. RT. Wash with PBS + 1 % BSA + 0.03 % saponine, resuspend in PBS.
Immunocytochemistry: Fixed and permeabilised cells. The antibody can distinguish TCR-stimulated from non-stimulated cells.

Restrictions: For Research Use only

Stockage

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Do not freeze.

Stock: 4 °C

Stockage commentaire: Store at 2-8°C. Do not freeze.

Références pour anticorps anti-CD3 epsilon (ABIN457415)

Tailor, Tsai, Shameli, Serra, Wang, Robbins, Nagata, Szymczak-Workman, Vignali, Santamaria: "The proline-rich sequence of CD3epsilon as an amplifier of low-avidity TCR signaling." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 181, Issue 1, pp. 243-55, (2008) (PubMed).

Wong, Dutly, Sacher, Lee, Hwang, Liu, Keshavjee, Hu, Waddell: "Targeted cell replacement with bone marrow cells for airway epithelial regeneration." dans: American journal of physiology. Lung cellular and molecular physiology, Vol. 293, Issue 3, pp. L740-52, (2007) (PubMed).

Rieux-Laucat, Hivroz, Lim, Mateo, Pellier, Selz, Fischer, Le Deist: "Inherited and somatic CD3zeta mutations in a patient with T-cell deficiency." dans: The New England journal of medicine, Vol. 354, Issue 18, pp. 1913-21, (2006) (PubMed).

Gil, Schrum, Alarcón, Palmer: "T cell receptor engagement by peptide-MHC ligands induces a conformational change in the CD3 complex of thymocytes." dans: The Journal of experimental medicine, Vol. 201, Issue 4, pp. 517-22, (2005) (PubMed).

Torres, Alcover, Zapata, Arnaud, Pacheco, Martín-Fernández, Villasevil, Sanal, Regueiro: "TCR dynamics in human mature T lymphocytes lacking CD3 gamma." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 170, Issue 12, pp. 5947-55, (2003) (PubMed).

Delgado, Fernández, Dave, Kappes, Alarcón: "CD3delta couples T-cell receptor signalling to ERK activation and thymocyte positive selection." dans: Nature, Vol. 406, Issue 6794, pp. 426-30, (2000) (PubMed).

Alarcón, Ley, Sánchez-Madrid, Blumberg, Ju, Fresno, Terhorst: "The CD3-gamma and CD3-delta subunits of the T cell antigen receptor can be expressed within distinct functional TCR/CD3 complexes." dans: The EMBO journal, Vol. 10, Issue 4, pp. 903-12, (1991) (PubMed).

Détails sur CD3 epsilon

Antigène: CD3 epsilon (CD3E)

Autre désignation: CD3 epsilon

Sujet: CD3 antigen, epsilon polypeptide,CD3 complex is crucial in transducing antigen-recognition signals into the cytoplasm of T cells and in regulating the cell surface expression of the TCR complex. T cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits CD3 gamma, CD3 delta, CD3 epsilon and CD3 zeta. These CD3 subunits are structurally related members of the immunoglobulins super family encoded by closely linked genes on human chromosome 11. The CD3 components have long cytoplasmic tails that associate with cytoplasmic signal transduction molecules. This association is mediated at least in part by a double tyrosine-based motif present in a single copy in the CD3 subunits. CD3 may play a role in TCR-induced growth arrest, cell survival and proliferation.,CD3 epsilon chain, T3E, IMD18

ID gène: 916

UniProt: P07766

Pathways: TCR Signaling, CXCR4-mediated Signaling Events, Ubiquitin Proteasome Pathway