EGFR anticorps (Internal Region)

N° du produit ABIN457474

Cet anticorps Mouton Polyclonal détecte spécifiquement EGFR dans WB, ELISA, IHC, IF, IHC (p), IP et ICC. Il présente une réactivité envers Humain, Souris, Rat et Poulet.

Aperçu rapide pour EGFR anticorps (Internal Region) (ABIN457474)

Antigène: EGFR (Epidermal Growth Factor Receptor (EGFR))

Reactivité: Humain, Souris, Rat, Poulet

Hôte: Mouton

Clonalité: Polyclonal

Conjugué: Cet anticorp EGFR est non-conjugé

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Détail du produit anti-EGFR anticorps

Épitope: Internal Region

Marque: IHC-plus™

Specificité: Recognizes human Epidermal Growth Factor Receptor.

Purification: Protein G purified

Immunogène: Recombinant 20aa residues representing a portion of the internal domain of EGFR proximal to the phosphorylation region.

Type of Immunogen: Recombinant protein

Isotype: IgG

Information d'application

Indications d'application: Approved: ELISA, ICC, IF, IHC, IHC-P (5 μg/mL), IP, WB

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Commentaires:

Target Species of Antibody: Human

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: Lot specific

Buffer: 0.15 M PBS,  pH 7.4. No preservatives added.

Agent conservateur: Without preservative

Conseil sur la manipulation: Avoid repeat freeze-thaw cycles.

Stock: 4 °C,-20 °C

Stockage commentaire: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Détails sur EGFR

Antigène: EGFR (Epidermal Growth Factor Receptor (EGFR))

Autre désignation: EGFR

Sujet: Name/Gene ID: EGFR
Subfamily: EGF Receptor
Family: Protein Kinase

Synonyms: EGFR, EGFf receptor, ERBB, ERBB1, MENA, PIG61, Proto-oncogene c-ErbB-1, V-erb-b homolog, EGF-R, HER1

ID gène: 1956

UniProt: P00533

Pathways: Signalisation NF-kappaB, Signalisation RTK, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Stem Cell Maintenance, Hepatitis C, Positive Regulation of Response to DNA Damage Stimulus, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, EGFR Downregulation, S100 Proteins