GABRA4 anticorps (AA 349-361)

N° du produit ABIN462297

Détail du produit anti-GABRA4 anticorps

Antigène: GABRA4 (gamma-aminobutyric Acid (GABA) A Receptor, alpha 4 (GABRA4))

Épitope: AA 349-361

Reactivité: Humain

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp GABRA4 est non-conjugé

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Marque: IHC-plus™

Specificité: Human GABRA4.

Homologie: Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey (100%) Marmoset, Elephant, Bovine (92%) Rabbit, Opossum (85%).

Purification: Immunoaffinity purified

Immunogène: Synthetic peptide C-EKAKRKTSKPPQE from an internal region of human GABRA4 (NP_000800.2). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey (100%), Marmoset, Elephant, Bovine (92%), Rabbit, Opossum (85%).

Type of Immunogen: Synthetic peptide

Information d'application

Indications d'application: Approved: IHC, IHC-P (2.5 μg/mL), Peptide-ELISA (1:16000), WB (0.1 - 0.3 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 2.5 μg/mL. Peptide ELISA: antibody detection limit dilution 1:16000. Western Blot: Approx. 70kD band observed in Human Brain (Cerebellum and Frontal Cortex) lysates (calculated MW of 61.6kD according to NP_000800.2). Recommended concentration: 0.1-0.3 μg/mL.

Commentaires:

Target Species of Antibody: Human

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Minimize freezing and thawing.

Stock: -20 °C

Stockage commentaire: Store at -20°C. Avoid freeze-thaw cycles.

Détails sur GABRA4

Antigène: GABRA4 (gamma-aminobutyric Acid (GABA) A Receptor, alpha 4 (GABRA4))

Autre désignation: GABRA4 (GABRA4 Produits)

Synonymes: anticorps GABRA4, anticorps Gabra-4, anticorps gamma-aminobutyric acid type A receptor alpha4 subunit, anticorps gamma-aminobutyric acid (GABA) A receptor, subunit alpha 4, anticorps GABRA4, anticorps Gabra4

Sujet: Name/Gene ID: GABRA4
Subfamily: GABA A receptor
Family: Ion Channel

Synonyms: GABRA4, GABA A receptor alpha 4, GABA(A) receptor, alpha 4

ID gène: 2557

NCBI Accession: NP_000800

UniProt: P48169

Pathways: Synaptic Membrane