SPI1 anticorps (AA 27-37)

N° du produit ABIN462506

Détail du produit anti-SPI1 anticorps

Antigène: SPI1 (Spleen Focus Forming Virus (SFFV) Proviral Integration Oncogene Spi1 (SPI1))

Épitope: AA 27-37

Reactivité: Humain, Porc, Chien, Boeuf (Vache), Cheval, Lapin

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp SPI1 est non-conjugé

Application: Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Marque: IHC-plus™

Specificité: Human SPI1 / PU.1. This antibody is expected to recognise both reported isoforms (NP_001074016.1, NP_003111.2).

Homologie: Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Panda, Bovine, Dog, Horse, Rabbit, Pig (100%) Marmoset, Opossum (91%) Rat, Platypus (82%).

Purification: Immunoaffinity purified

Immunogène: Synthetic peptide C-DLYQRQTHEYY from an internal region of human SPI1 / PU.1 (NP_001074016.1, NP_003111.2). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Panda, Bovine, Dog, Horse, Rabbit, Pig (100%), Marmoset, Opossum (91%), Rat, Platypus (82%).

Type of Immunogen: Synthetic peptide

Information d'application

Indications d'application: Approved: IHC, IHC-P (3.75 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 3.75 μg/mL.

Commentaires:

Target Species of Antibody: Human

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Minimize freezing and thawing.

Stock: -20 °C

Stockage commentaire: Store at -20°C. Avoid freeze-thaw cycles.

Détails sur SPI1

Antigène: SPI1 (Spleen Focus Forming Virus (SFFV) Proviral Integration Oncogene Spi1 (SPI1))

Autre désignation: SPI1 / PU.1 (SPI1 Produits)

Synonymes: anticorps OF, anticorps PU.1, anticorps SFPI1, anticorps SPI-1, anticorps SPI-A, anticorps Dis-1, anticorps Dis1, anticorps Sfpi-1, anticorps Sfpi1, anticorps Spi-1, anticorps Tcfpu1, anticorps Tfpu.1, anticorps Pu.1, anticorps cb1086, anticorps pu.1, anticorps pu1, anticorps sfpi1, anticorps si:by184l24.2, anticorps spi1, anticorps SPI1/PU.1, anticorps spi1l, anticorps zgc:152991, anticorps Spi-1 proto-oncogene, anticorps spleen focus forming virus (SFFV) proviral integration oncogene, anticorps Spi-1 proto-oncogene b, anticorps Spi-1 proto-oncogene a, anticorps SPI1, anticorps Spi1, anticorps spi1b, anticorps spi1a

Sujet: Name/Gene ID: SPI1

Synonyms: SPI1, 31 kDa-transforming protein, 31 kDa transforming protein, PU.1, SFPI1, SPI-1, SPI-1 proto-oncogene, SPI-A, Transcription factor PU.1

ID gène: 6688

NCBI Accession: NP_001074016, NP_003111

UniProt: P17947

Pathways: Stem Cell Maintenance