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IL12 anticorps

Cet anticorps Chèvre Polyclonal détecte spécifiquement IL12 dans ELISA, WB et Neut. Il présente une réactivité envers Humain.
N° du produit ABIN465439

Aperçu rapide pour IL12 anticorps (ABIN465439)

Antigène

Voir toutes IL12 Anticorps
IL12 (Interleukin 12 (IL12))

Reactivité

  • 54
  • 26
  • 16
  • 7
  • 4
  • 2
  • 1
  • 1
Humain

Hôte

  • 33
  • 31
  • 19
  • 16
  • 2
Chèvre

Clonalité

  • 50
  • 48
Polyclonal

Conjugué

  • 48
  • 16
  • 4
  • 4
  • 4
  • 3
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 1
Cet anticorp IL12 est non-conjugé

Application

  • 75
  • 63
  • 26
  • 26
  • 16
  • 12
  • 11
  • 9
  • 8
  • 7
  • 7
  • 6
  • 4
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
ELISA, Western Blotting (WB), Neutralization (Neut)
  • Specificité

    Human IL-12

    Purification

    Immunoaffinity purified

    Immunogène

    CHO cell derived recombinant Human IL-12.

    Type of Immunogen: Cell extract
  • Indications d'application

    Approved: ELISA (0.5 - 2 μg/mL), Neut (0.01 - 0.02 μg/mL), WB

    Usage: Neutralization: To yield one-half maximal inhibition [ND] of the biological activity of hIL-12 (0.3 ng/mL), a concentration of 012-02 μg/mL of this antibody is required. ELISA: To detect hIL-12 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5-2 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human IL-12 (LS-C104425) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hIL-12. Western Blot: To detect hIL-12 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIL-12 is 1.5-3 ng/lane, under either reducing or non-reducing conditions.

    Commentaires

    Target Species of Antibody: Human

    Restrictions

    For Research Use only
  • Format

    Lyophilized

    Reconstitution

    Sterile water

    Concentration

    Lot specific

    Buffer

    Lyophilized from PBS,  pH 7.2

    Conseil sur la manipulation

    Avoid freeze/thaw cycles.

    Stock

    4 °C,-20 °C

    Stockage commentaire

    Store Lyophilized at room temperature up to 1 month
    Reconstituted for up to 2 weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.
  • Antigène

    IL12 (Interleukin 12 (IL12))

    Autre désignation

    IL12 / IL-12

    Sujet

    Name/Gene ID: IL-12

    Synonyms: IL-12

    Pathways

    Signalistation JAK/STAT, Signalisation TLR, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Activated T Cell Proliferation, Cancer Immune Checkpoints, Inflammasome
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