IL1A anticorps
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- Antigène Voir toutes IL1A Anticorps
- IL1A (Interleukin 1 alpha (IL1A))
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Reactivité
- Humain
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Hôte
- Lapin
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Clonalité
- Polyclonal
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Conjugué
- Cet anticorp IL1A est non-conjugé
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Application
- Western Blotting (WB), ELISA, Neutralization (Neut)
- Specificité
- Human IL-1alpha
- Purification
- Immunoaffinity purified
- Immunogène
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E.coli derived recombinant Human IL-1alpha.
Type of Immunogen: Cell extract - Top Product
- Discover our top product IL1A Anticorps primaire
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- Indications d'application
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Approved: ELISA, Neut (1 - 2 μg/mL), WB (0.1 - 0.2 μg/mL)
Usage: Immunohistochemistry: This antibody stained formalin-fixed, paraffin-embedded sections of normal human skin. The recommended concentration is 1 μg/mL overnight at 4 °C. An HRP-labeled polymer detection system was used with a non-alcohol soluble AEC chromogen. Enzyme induced antigen retrieval was performed with proteinase K. Optimal concentrations and conditions may vary. Neutralization: To yield one-half maximal inhibition [ND] of the biological activity of hIL-1alpha (20 ng/mL), a concentration of 1-2 μg/mL of this antibody is required. ELISA: To detect hIL-1alpha by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5-2 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human IL-1alpha (LS-C104520) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hIL-1alpha. Western Blot: To detect hIL-1alpha by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIL-1alpha is 1.5-3 ng/lane, under either reducing or non-reducing conditions. - Commentaires
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Target Species of Antibody: Human
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Sterile water
- Concentration
- Lot specific
- Buffer
- Lyophilized from PBS, pH 7.2
- Conseil sur la manipulation
- Avoid freeze/thaw cycles.
- Stock
- 4 °C,-20 °C
- Stockage commentaire
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Store Lyophilized at room temperature up to 1 month
Reconstituted for up to 2 weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.
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- Antigène
- IL1A (Interleukin 1 alpha (IL1A))
- Autre désignation
- IL1A / IL1 Alpha (IL1A Produits)
- Synonymes
- anticorps IL-1A, anticorps IL1, anticorps IL1-ALPHA, anticorps IL1F1, anticorps IL-6, anticorps Il-1a, anticorps IL-1 alpha, anticorps IL-1alpha, anticorps L1A, anticorps interleukin 1 alpha, anticorps interleukin 6, anticorps IL1A, anticorps IL6, anticorps Il1a
- Sujet
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Name/Gene ID: IL1A
Family: IL-1
Synonyms: IL1A, Hematopoietin-1, IL-1 alpha, IL1, IL1-ALPHA, IL1 Alpha, Interleukin-1 alpha, IL-1A, IL1F1, Pro-interleukin-1-alpha, Preinterleukin 1 alpha, Interleukin 1, alpha - ID gène
- 3552
- UniProt
- P01583
- Pathways
- Signalisation NF-kappaB, Autophagy, Cancer Immune Checkpoints
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