IL1A anticorps
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- Antigène Voir toutes IL1A Anticorps
- IL1A (Interleukin 1 alpha (IL1A))
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Reactivité
- Souris
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Hôte
- Lapin
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Clonalité
- Polyclonal
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Conjugué
- Cet anticorp IL1A est non-conjugé
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Application
- Western Blotting (WB), ELISA, Neutralization (Neut)
- Specificité
- Mouse IL-1alpha
- Purification
- Immunoaffinity purified
- Immunogène
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E.coli derived recombinant Murine IL-1alpha.
Type of Immunogen: Cell extract - Top Product
- Discover our top product IL1A Anticorps primaire
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- Indications d'application
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Approved: ELISA (0.5 - 2 μg/mL), Neut (0.1 μg/mL), WB
Usage: Immunohistochemistry: This antibody stained colchicine injected mouse brain (including the hippocampal fissure) tissue. The primary antibody was incubated at 1 mg/mL overnight at 4 °C. This was followed by a peroxidase conjugated secondary antibody and then a fluorescein Tyramide Signal Amplification (TSA) reagent. Optimal concentrations and conditions may vary. Neutralization: To yield one-half maximal inhibition [ND] of the biological activity of mIL-1alpha (0.05 ng/mL), a concentration of 0 3-0.05 μg/mL of this antibody is required. ELISA: To detect mIL-1alpha by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5-2 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Murine IL-1alpha (LS-C104815) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant mIL-1alpha. Western Blot: To detect mIL-1alpha by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant mIL-1alpha is 1.5-3 ng/lane, under either reducing or non-reducing conditions. - Commentaires
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Target Species of Antibody: Mouse
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Sterile water
- Concentration
- Lot specific
- Buffer
- Lyophilized from PBS, pH 7.2
- Stock
- 4 °C,-20 °C
- Stockage commentaire
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Store Lyophilized at room temperature up to 1 month
Reconstituted for up to 2 weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.
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- Antigène
- IL1A (Interleukin 1 alpha (IL1A))
- Autre désignation
- IL1A (IL1A Produits)
- Synonymes
- anticorps IL-1A, anticorps IL1, anticorps IL1-ALPHA, anticorps IL1F1, anticorps IL-6, anticorps Il-1a, anticorps IL-1 alpha, anticorps IL-1alpha, anticorps L1A, anticorps interleukin 1 alpha, anticorps interleukin 6, anticorps IL1A, anticorps IL6, anticorps Il1a
- Sujet
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Name/Gene ID: IL1A
Family: IL-1
Synonyms: IL1A, Hematopoietin-1, IL-1 alpha, IL1, IL1-ALPHA, IL1 Alpha, Interleukin-1 alpha, IL-1A, IL1F1, Pro-interleukin-1-alpha, Preinterleukin 1 alpha, Interleukin 1, alpha - ID gène
- 3552
- Pathways
- Signalisation NF-kappaB, Autophagy, Cancer Immune Checkpoints
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