PDGF-AA Homodimer anticorps (Biotin)

N° du produit ABIN465591

Détail du produit

Antigène: PDGF-AA Homodimer

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Biotin

Application: Western Blotting (WB), ELISA

Specificité: Human PDGF-AA

Purification: Immunoaffinity purified

Immunogène: E.coli derived recombinant Human PDGF-AA.

Type of Immunogen: Cell extract

Information d'application

Indications d'application: Approved: ELISA (1 μg/mL), WB (0.1 - 0.2 μg/mL)

Usage: ELISA: To detect hPDGF-AA by direct ELISA (using 100 μL/well antibody solution) a concentration of 0.25-1 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2-0.4 ng/well of recombinant hPDGF-AA. To detect hPDGF-AA by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25-1 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with Polyclonal Anti-Human PDGF-AA (LS-B7488) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hPDGF-AA. Western Blot: To detect hPDGF-AA by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hPDGF-AA is 1.5-3 ng/lane, under either reducing or non-reducing conditions. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

Commentaires:

Target Species of Antibody: Human

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Sterile PBS containing 0.1 % BSA

Concentration: Lot specific

Buffer: Lyophilized from PBS,  pH 7.2

Conseil sur la manipulation: Avoid freeze/thaw cycles.

Stock: 4 °C,-20 °C

Stockage commentaire: Store Lyophilized at room temperature up to 1 month
Reconstituted for up to 2 weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.

Détail du antigène

Antigène: PDGF-AA Homodimer

Autre désignation: PDGF-AA

Synonymes: anticorps PDGF-A, anticorps PDGF1, anticorps AA409063, anticorps CD142, anticorps Cf-3, anticorps Cf3, anticorps TF, anticorps PDGFACP, anticorps platelet derived growth factor subunit A, anticorps coagulation factor III, anticorps platelet derived growth factor, alpha, anticorps PDGFA, anticorps F3, anticorps Pdgfa

Sujet: Name/Gene ID: PDGFA
Family: PDGF

Synonyms: PDGFA, PDGF-A, PDGF A-chain, PDGF subunit A, PDGF-1, PDGF1

ID gène: 5154

UniProt: P04085