TNF alpha anticorps
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- Antigène Voir toutes TNF alpha Anticorps
- TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))
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Reactivité
- Humain
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Hôte
- Souris
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Clonalité
- Monoclonal
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Conjugué
- Cet anticorp TNF alpha est non-conjugé
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Application
- ELISA, Neutralization (Neut)
- Specificité
- Human TNF-alpha
- Purification
- Ion exchange chromatography
- Immunogène
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E.coli derived recombinant Human TNF-alpha.
Type of Immunogen: Cell extract - Isotype
- IgG1
- Top Product
- Discover our top product TNF alpha Anticorps primaire
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- Indications d'application
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Approved: ELISA (4 - 8 μg/mL), Neut (50 - 55 μg/mL), SwELISA
Usage: Immunohistochemistry: This antibody stained formalin-fixed, paraffin-embedded sections of human breast invasive ductal carcinoma. The recommended concentrations are 15-20 μg/mL overnight at 4 °C. An HRP-labeled polymer detection system was used with DAB Chromogen. Heat induced antigen retrieval with a pH 6 sodium citrate buffer is recommended. Optimal concentrations and conditions may vary. Tissue samples were provided by the Cooperative Human Tissue Network, which is funded by the National Cancer Institute. *Tissue samples were provided by the Cooperative Human Tissue Network, which is funded by the National Cancer Institute. Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of Human TNF-alpha (0.5 ng/mL), a concentration of < 0.1 μg/mL of this antibody is required. ELISA: In a sandwich ELISA (assuming 100 μL/well), a concentration of 2-4 μg/mL of this antibody will detect at least 1000 pg/mL of recombinant Human TNF-alpha when used with biotinylated antigen affinity purified anti-Human TNF-alpha (LS-C104719) as the detection antibody at a concentration of approximately 0.5-1 μg/mL. Western Blot: To detect Human TNF-alpha by Western Blot analysis this antibody can be used at a concentration of 0.5-1 μg/mL. When used in conjunction with compatible secondary reagents the detection limit for recombinant Human TNF-alpha is 0.5-1 ng/lane, under reducing conditions and 0.25-0.50 ng/lane, under non-reducing conditions. - Commentaires
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Target Species of Antibody: Human
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Sterile water
- Concentration
- Lot specific
- Buffer
- Lyophilized from PBS
- Conseil sur la manipulation
- Avoid freeze/thaw cycles.
- Stock
- 4 °C,-20 °C
- Stockage commentaire
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Store Lyophilized at room temperature up to 1 month
Reconstituted for up to 2 weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.
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- Antigène
- TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))
- Autre désignation
- TNF Alpha (TNF alpha Produits)
- Synonymes
- anticorps DIF, anticorps TNF-alpha, anticorps TNFA, anticorps TNFSF2, anticorps RATTNF, anticorps Tnfa, anticorps tnf, anticorps TNF-a, anticorps TNFalpha, anticorps Tnfsf1a, anticorps TNFa, anticorps cTNF, anticorps Tnf-alpha, anticorps tnfa-like, anticorps TNF-ALPHA, anticorps dif, anticorps tnfa, anticorps xtnf, anticorps tnfsf2, anticorps tnf-alpha, anticorps Cachectin, anticorps tumor necrosis factor, anticorps tumor necrosis factor b (TNF superfamily, member 2), anticorps tumor necrosis factor alpha, anticorps tumor necrosis factor a (TNF superfamily, member 2), anticorps TNF, anticorps Tnf, anticorps tnf, anticorps tnfb, anticorps tnf-alpha, anticorps LOC103694380, anticorps tnfa
- Sujet
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Name/Gene ID: TNF
Family: TNF
Synonyms: TNF, APC1 protein, Cachectin, DIF, TNF Alpha, Tumor necrosis factor-alpha, TNF-alpha, TNF-a, TNFA, Tumor necrosis factor, TNF, macrophage-derived, TNF, monocyte-derived, TNFSF2 - ID gène
- 7124
- UniProt
- P01375
- Pathways
- Signalisation NF-kappaB, Apoptose, Caspase Cascade in Apoptosis, Signalisation TLR, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Inflammasome
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