CNTF anticorps

N° du produit ABIN465749

Détail du produit anti-CNTF anticorps

Antigène: CNTF (Ciliary Neurotrophic Factor (CNTF))

Reactivité: Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp CNTF est non-conjugé

Application: Western Blotting (WB), ELISA, Neutralization (Neut)

Specificité: Rat CNTF

Purification: Immunoaffinity purified

Immunogène: E.coli derived recombinant Rat CNTF.

Type of Immunogen: Cell extract

Information d'application

Indications d'application: Approved: ELISA (0.5 - 2 μg/mL), Neut (2 - 5 μg/mL), WB

Usage: Immunohistochemistry: This antibody stained colchicine injected rat brain (including the globus pallidus region) tissue. The primary antibody was incubated at 0.25 mg/mL overnight at 4 °C. This was followed by a peroxidase conjugated secondary antibody and then a fluorescein Tyramide Signal Amplification (TSA) reagent. Optimal concentrations and conditions may vary. Information and photo are courtesy of the Tissue Profiling group, SciLifeLab Stockholm. Neutralization: To yield one-half maximal inhibition [ND] of the biological activity of Rat CNTF (0.15 ng/mL), a concentration of 0.02-0.05 μg/mL of this antibody is required. ELISA: To detect Rat CNTF by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5-2 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Rat CNTF (LS-C104736) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant Rat CNTF. Western Blot: To detect Rat CNTF by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant Rat CNTF is 1.5-3 ng/lane, under either reducing or non-reducing conditions.

Commentaires:

Target Species of Antibody: Rat

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Sterile water

Concentration: Lot specific

Buffer: Lyophilized from PBS,  pH 7.2

Conseil sur la manipulation: Avoid freeze/thaw cycles.

Stock: 4 °C,-20 °C

Stockage commentaire: Store Lyophilized at room temperature up to 1 month
Reconstituted for up to 2 weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.

Détails sur CNTF

Antigène: CNTF (Ciliary Neurotrophic Factor (CNTF))

Autre désignation: CNTF (CNTF Produits)

Synonymes: anticorps GPA, anticorps CNTF, anticorps HCNTF, anticorps AI429687, anticorps ciliary neurotrophic factor, anticorps CNTF, anticorps Cntf

Sujet: Name/Gene ID: CNTF

Synonyms: CNTF, Ciliary neurotrophic factor, HCNTF

ID gène: 1270

UniProt: P26441

Pathways: Signalistation JAK/STAT