KDELR1 anticorps (C-Term)

N° du produit ABIN487508

Détail du produit anti-KDELR1 anticorps

Antigène: KDELR1 (KDEL (Lys-Asp-Glu-Leu) Endoplasmic Reticulum Protein Retention Receptor 1 (KDELR1))

Épitope: C-Term

Reactivité: Drosophila melanogaster, Plantes, Saccharomyces cerevisiae

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp KDELR1 est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF)

Specificité: Detects ~78 kDa protein. The 2E7 clone recognizes the C-terminal peptide HDEL, a common version of the endoplasmic reticulum retention signal found in Yeast, Plant, nematode and other ER proteins. It specifically stains HDEL proteins in barnyard grass, beet, cotton, mung bean, sorghum and wheat (4).

Réactivité croisée (Details): Species reactivity (tested):Drosophila, Yeast, Saccharomyce, Plants (barnyard grass, beet, cotton, mung bean, sorghum and wheat).

Attributs du produit: Synonyms: KDEL endoplasmic reticulum protein retention receptor 1, ERD2.1, ER marker, ER lumenprotein retaining receptor 1, Putative MAPK-activating protein PM23

Purification: Protein G Chromatography.

Immunogène: A synthetic HDEL peptide corresponding to the C-terminus of Yeast Bip.

Clone: 2-00E-007

Isotype: IgG2b

Information d'application

Indications d'application: Western blot: 1/1000-1/2000.1 μg/mL of SMC-175 was sufficient for detection of HDEL-containing proteins in 10 μg of S. cerevisiae lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG -HRP asthe secondary antibody. Immunofluorescence: 1/50-1/500.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL

Buffer: PBS, pH 7.4 containing 50 % Glycerol with 0.09 % Sodium Azide as preservative.

Agent conservateur: Sodium azide

Précaution d'utilisation: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -28 °C for longer.
Shelf life: one year from despatch.

Date de péremption: 12 months

Détails sur KDELR1

Antigène: KDELR1 (KDEL (Lys-Asp-Glu-Leu) Endoplasmic Reticulum Protein Retention Receptor 1 (KDELR1))

Autre désignation: KDEL Receptor 1 / KDELR1 (KDELR1 Produits)

Synonymes: anticorps ERD2, anticorps ERD2.1, anticorps HDEL, anticorps PM23, anticorps 8030486F04Rik, anticorps AW215843, anticorps Erd2.1, anticorps KDEL endoplasmic reticulum protein retention receptor 1, anticorps KDEL (Lys-Asp-Glu-Leu) endoplasmic reticulum protein retention receptor 1, anticorps KDELR1, anticorps Kdelr1

Sujet: HSP 70 family comprises four highly conserved proteins, HSP 70, HSC 70, GRP 75 and GRP 78, which serve a variety of roles. They act as molecular chaperones, facilitating the assembly of multi-protein complexes, participate in the translocation of polypeptides across cell membranes and to the nucleus, and aid in the proper folding of nascent polypeptide chains (1, 2). GRP 78 is localized in the endoplasmic reticulum (ER), where it receives imported secretory proteins and is involved in the folding and translocation of nascent peptide chains (2). Sorting of these proteins is dependent on a C-terminal tetrapeptide signal, usually KDEL in animal cells, and HDEL in S.cerevisiae (3).Synonyms: ER lumen protein retaining receptor 1, ER marker, ERD2.1, KDEL endoplasmic reticulum protein retention receptor 1, Putative MAPK-activating protein PM23

ID gène: 10945

UniProt: P24390

Pathways: Maintenance of Protein Location